Cortela Denise Costa Boamorte, Nogueira Maria Renata Sales, Pereira Ana Carla, Souza Junior Alcione Lescano de, Ignotti Eliane
Universidade do Estado de Mato Grosso, Faculdade de Ciências da Saúde, Cáceres, Mato Grosso, Brazil.
Instituto Lauro de Souza Lima, Departamento de Pesquisa, Bauru, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2018 Nov 8;60:e68. doi: 10.1590/S1678-9946201860068.
The inflammatory cytokines involved in the immune response to chronic periodontal disease (CPD) in the context of leprosy reactions (LR) were analyzed in 57 new cases of multibacillary leprosy (MBL). They were stratified by the presence of CPD and LR. Messenger RNA (mRNA) expression of inflammatory mediators was determined by qRT-PCR using skin biopsy and by ELISA using serum samples, maintaining 5% of significance level in ANOVA and correlation analyses. Twenty-three (40.4%) patients presented the first LR, whereas 22 (45.0%) patients presented CPD. IL-4 and IL-6 serum levels were significantly lower in patients with CPD and LR than in patients without CPD but with LR; IFN-γ serum levels were higher in patients with CPD and LR than in patients with no CPD and no LR; IL-4 serum levels were negatively correlated with TNF-α gene expression, while IL-6 serum levels were positively correlated with IFN-γ gene expression, in the skin of subjects with CPD and LR. The presence of DPC in individuals with LR immunoregulated IL-6, IFN-γ, and IL-4 concentrations. The presence of DPC decreased serum levels of IL-6 and IL-4 in reactional individuals. CPD concomitant to LR resulted in increased IFN-γ serum levels.
在57例新确诊的多菌型麻风（MBL）病例中，分析了麻风反应（LR）背景下参与慢性牙周病（CPD）免疫反应的炎性细胞因子。根据是否存在CPD和LR对患者进行分层。使用皮肤活检通过qRT-PCR以及使用血清样本通过ELISA测定炎性介质的信使核糖核酸（mRNA）表达，在方差分析和相关性分析中保持5%的显著性水平。23例（40.4%）患者出现首次LR，而22例（45.0%）患者患有CPD。CPD合并LR患者的IL-4和IL-6血清水平显著低于无CPD但有LR的患者；CPD合并LR患者的IFN-γ血清水平高于无CPD且无LR的患者；在CPD合并LR患者的皮肤中，IL-4血清水平与TNF-α基因表达呈负相关，而IL-6血清水平与IFN-γ基因表达呈正相关。LR患者中DPC的存在对IL-6、IFN-γ和IL-4浓度具有免疫调节作用。DPC的存在降低了反应期患者的IL-6和IL-4血清水平。CPD合并LR导致IFN-γ血清水平升高。
