Kanno Claudia Misue, Oliveira Jose Americo de, Ervolino Edilson, Soubhia Ana Maria Pires
Univ. Estadual Paulista, Faculdade de Odontologia, Departamento de Emergência, Araçatuba, São Paulo, Brasil.
Univ. Estadual Paulista, Faculdade de Odontologia, Departamento de Ciências Básicas, Araçatuba, São Paulo, Brasil.
J Appl Oral Sci. 2018 Nov 8;27:e20180135. doi: 10.1590/1678-7757-2018-0135.
Myofibroblasts have been associated with the development of several pathologic fibrotic conditions. This longitudinal study aims to assess the proliferative and antiapoptotic effects of cyclosporin, nifedipine and phenytoin on gingival connective tissue cells of nonhuman primate, as well as to analyze a possible role of myofibroblasts in gingival overgrowth.
Gingival samples from the right superior canine area were obtained from 12 male monkeys ( Sapajus spp ) to comprise the control group. After one week, the animals were randomly assigned to three groups, which received daily oral doses of cyclosporin, nifedipine or phenytoin for 120 days. Gingival samples were collected from the left superior canine area of two animals of each group at 52 and 120 days. Histological sections were stained with hematoxylin and eosin, and immunoreacted against α-SMA, Ki- 67 and bcl-2.
α-SMA immunoreaction was negative in the control and experimental groups. Similarly, no difference between groups concerning immunostaining against Ki-67 and bcl-2 was observed in connective tissue cells.
Based on this methodology, it may be concluded that gingival overgrowths induced by cyclosporin, nifedipine and phenytoin are not associated with neither myofibroblast transdifferentiation, proliferation nor apoptosis of gingival connective cells in monkeys.
肌成纤维细胞与多种病理性纤维化疾病的发展有关。这项纵向研究旨在评估环孢素、硝苯地平和苯妥英对非人灵长类动物牙龈结缔组织细胞的增殖和抗凋亡作用，并分析肌成纤维细胞在牙龈过度生长中可能发挥的作用。
从12只雄性猴子（僧面猴属）的右上犬齿区域获取牙龈样本，组成对照组。一周后，将动物随机分为三组，分别每日口服环孢素、硝苯地平或苯妥英，持续120天。在第52天和第120天，从每组的两只动物的左上犬齿区域采集牙龈样本。组织学切片用苏木精和伊红染色，并与α-SMA、Ki-67和bcl-2进行免疫反应。
对照组和实验组的α-SMA免疫反应均为阴性。同样，在结缔组织细胞中，各组之间针对Ki-67和bcl-2的免疫染色也未观察到差异。
基于该方法，可以得出结论，环孢素、硝苯地平和苯妥英诱导的牙龈过度生长与猴子牙龈结缔组织细胞的肌成纤维细胞转分化、增殖或凋亡均无关。
