Olimpio Eduardo P, Dang Yiteng, Youk Hyun
Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands; Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands.
Kavli Institute of Nanoscience, Delft University of Technology, Delft, the Netherlands; Department of Bionanoscience, Delft University of Technology, Delft 2629HZ, the Netherlands.
iScience. 2018 Apr 27;2:27-40. doi: 10.1016/j.isci.2018.03.013. Epub 2018 Apr 6.
Communicating cells can coordinate their gene expressions to form spatial patterns, generating order from disorder. Ubiquitous "secrete-and-sense cells" secrete and sense the same molecule to do so. Here we present a modeling framework-based on cellular automata and mimicking approaches of statistical mechanics-for understanding how secrete-and-sense cells with bistable gene expression, from disordered beginnings, can become spatially ordered by communicating through rapidly diffusing molecules. Classifying lattices of cells by two "macrostate" variables-"spatial index," measuring degree of order, and average gene-expression level-reveals a conceptual picture: a group of cells behaves as a single particle, in an abstract space, that rolls down on an adhesive "pseudo-energy landscape" whose shape is determined by cell-cell communication and an intracellular gene-regulatory circuit. Particles rolling down the landscape represent cells becoming more spatially ordered. We show how to extend this framework to more complex forms of cellular communication.
进行通信的细胞能够协调它们的基因表达以形成空间模式，从无序中产生秩序。无处不在的“分泌与感知细胞”通过分泌和感知相同的分子来实现这一点。在此，我们提出一个基于细胞自动机并模仿统计力学方法的建模框架，用于理解具有双稳态基因表达的分泌与感知细胞如何从无序状态开始，通过快速扩散分子进行通信而实现空间有序。通过两个“宏观状态”变量——测量有序程度的“空间指数”和平均基因表达水平——对细胞晶格进行分类，揭示了一个概念性图景：一组细胞在抽象空间中表现为单个粒子，该粒子在由细胞间通信和细胞内基因调控回路决定形状的粘性“伪能量景观”上滚落。沿景观滚落的粒子代表细胞在空间上变得更加有序。我们展示了如何将此框架扩展到更复杂的细胞通信形式。
