Biswal Manisha, Zaman Kamran, Suri Vikas, Rao Harshith, Kumar Abhay, Kapur Gaurav, Sharma Navneet, Bhalla Ashish, Jayashree M
Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Med Microbiol. 2018 Jul-Sep;36(3):422-425. doi: 10.4103/ijmm.IJMM_18_8.
Scrub typhus caused by Orientia tsutsugamushi presents as an acute febrile illness with a varied presentation from mild illness to fatal disease in the absence of appropriate antibiotic treatment. Performing polymerase chain reaction (PCR) on eschar sample acts a rapid diagnostic tool in the early stage of scrub typhus when blood is negative. A total of eight patients from whom both whole blood and eschar samples were collected and tested by nested PCR targeting 56 kDa trichostatin A (TSA) gene to detect O. tsutsugamushi DNA. All (100%) eschar samples and three whole blood samples tested positive. Genetic analysis of the 56 kDa TSA gene sequences showed that the majority were related to Karp reference strains, while one clustered with Kawasaki strain. When present, eschar should be favoured as a diagnostic sample over whole blood in the early phase of infection.
由恙虫病东方体引起的恙虫病表现为一种急性发热性疾病，在没有适当抗生素治疗的情况下，其表现从轻度疾病到致命疾病各不相同。在焦痂样本上进行聚合酶链反应（PCR）是恙虫病早期血液检测为阴性时的一种快速诊断工具。共有8名患者同时采集了全血和焦痂样本，并通过针对56 kDa曲古抑菌素A（TSA）基因的巢式PCR检测恙虫病东方体DNA。所有（100%）焦痂样本和3份全血样本检测呈阳性。对56 kDa TSA基因序列的遗传分析表明，大多数与Karp参考菌株相关，而其中一个与川崎菌株聚类。在感染早期，若有焦痂存在，应优先将其作为诊断样本而非全血。
