Kala Deepak, Sharma Tarun Kumar, Gupta Shagun, Nagraik Rupak, Verma Vivek, Thakur Atul, Kaushal Ankur
Amity Center of Nanotechnology, Amity University, Haryana, 122413 India.
Translational Health Science, and Technology Institute, Faridabad, 121001 India.
3 Biotech. 2020 Oct;10(10):446. doi: 10.1007/s13205-020-02432-w. Epub 2020 Sep 19.
A novel approach has been developed for the detection of - (TSA) gene of a causative agent of scrub typhus disease. The approach was developed by immobilization of 5' NH2 labeled ssDNA probe selective gene, to the surface of AuNPs/CNF modified screen-printed electrode. An electrochemical response was recorded with single stranded genomic DNA (ssDNA) of isolated from patient sample, using cyclic voltammetry and electrochemical impedance spectroscopy. The electrode surface was characterized by Field-Emission Scanning electron microscope (FE-SEM), Fourier Transform Infrared Spectroscopy (FTIR) and Raman Spectroscopy at each step of fabrication. The DNA biosensor shows optimum response within 50-60 s at room temperature (25 ± 3 °C). The sensor shows higher sensitivity [7849 (µA/cm)/ng DNA], fast response time (60 s), wider linear range (0.04-2.6 ng) with limit of detection of 0.02 ng/µl of ssDNA sample.
已开发出一种用于检测恙虫病病原体-（TSA）基因的新方法。该方法是通过将5' NH2标记的选择性ssDNA探针固定在AuNPs/CNF修饰的丝网印刷电极表面来实现的。使用循环伏安法和电化学阻抗谱对从患者样本中分离出的单链基因组DNA（ssDNA）记录电化学响应。在制造的每个步骤中，通过场发射扫描电子显微镜（FE-SEM）、傅里叶变换红外光谱（FTIR）和拉曼光谱对电极表面进行表征。该DNA生物传感器在室温（25±3°C）下50-60秒内显示出最佳响应。该传感器具有更高的灵敏度[7849（µA/cm）/ng DNA]、快速响应时间（60秒）、更宽的线性范围（0.04-2.6 ng）以及0.02 ng/µl ssDNA样品的检测限。
