Choi Ja Eun, Kim Somi, Lee Jisu, Kim Kyungjin, Kaang Bong-Kiun
School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
Department of Brain and Cognitive Sciences, DGIST, Daegu 42988, Korea.
Exp Neurobiol. 2018 Oct;27(5):344-349. doi: 10.5607/en.2018.27.5.344. Epub 2018 Oct 31.
Circadian rhythms are driven by circadian oscillators, and these rhythms result in the biological phenomenon of 24-h oscillations. Previous studies suggest that learning and memory are affected by circadian rhythms. One of the genes responsible for generating the circadian rhythm is Rev-erbα. The REV-ERBα protein is a nuclear receptor that acts as a transcriptional repressor, and is a core component of the circadian clock. However, the role of REV-ERBα in neurophysiological processes in the hippocampus has not been characterized yet. In this study, we examined the time-dependent role of REV-ERBα in hippocampal synaptic plasticity using Rev-erbα KO mice. The KO mice lacking REV-ERBα displayed abnormal NMDAR-dependent synaptic potentiation (E-LTP) at CT12CT14 (subjective night) when compared to their wild-type littermates. However, Rev-erbα KO mice exhibited normal E-LTP at CT0CT2 (subjective day). We also found that the Rev-erbα KO mice had intact late LTP (L-LTP) at both subjective day and night. Taken together, these results provide evidence that REV-ERBα is critical for hippocampal E-LTP during the dark period.
昼夜节律由昼夜振荡器驱动，这些节律导致了24小时振荡的生物学现象。先前的研究表明，学习和记忆受昼夜节律影响。负责产生昼夜节律的基因之一是Rev-erbα。REV-ERBα蛋白是一种核受体，作为转录抑制因子，是生物钟的核心组成部分。然而，REV-ERBα在海马体神经生理过程中的作用尚未明确。在本研究中，我们使用Rev-erbα基因敲除小鼠研究了REV-ERBα在海马体突触可塑性中的时间依赖性作用。与野生型同窝小鼠相比，缺乏REV-ERBα的基因敲除小鼠在CT12CT14（主观夜间）表现出异常的NMDAR依赖性突触增强（E-LTP）。然而，Rev-erbα基因敲除小鼠在CT0CT2（主观白天）表现出正常的E-LTP。我们还发现，Rev-erbα基因敲除小鼠在主观白天和夜间均具有完整的晚期LTP（L-LTP）。综上所述，这些结果证明REV-ERBα在黑暗期对海马体E-LTP至关重要。
