Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany.
Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany.
Biochem Med (Zagreb). 2018 Oct 15;28(3):030703. doi: 10.11613/BM.2018.030703.
Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS.
We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I (ß2GPI) antibodies.
The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034). When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of ß2GPI and CL IgG and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS "criteria" antibody against CL (P = 0.764).
We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.
针对 Annexin A8（AnxA8）的抗体尚未在患有抗磷脂综合征（APS）的患者中进行研究。本研究旨在比较 APS 患者和年龄匹配的健康对照组血清中 AnxA8 抗体的存在，并探讨 AnxA8 抗体是否是 APS 的潜在生物标志物。
在这项病例对照研究中，我们纳入了 22 名 APS 患者和 22 名健康对照者。我们使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳和免疫印迹来检测 AnxA8 抗体的存在，并应用酶联免疫吸附试验来检测心磷脂（CL）和β-2-糖蛋白 I（ß2GPI）抗体的存在。
与 22 名健康对照组的血清相比，22 名 APS 患者中有 9 名的血清显示出 AnxA8 IgG 同种型抗体反应性（P = 0.034）。当我们也包括弱免疫印迹信号时，与 22 名健康对照组的 3 名相比，12 名 APS 患者表现出 AnxA8 IgG 同种型抗体反应性（P = 0.005）。我们还研究了 APS 患者血清中 AnxA8 IgM 同种型抗体的存在，但未发现 APS 患者组与健康对照组之间存在统计学上的显著差异（P = 0.500）。我们进一步研究了ß2GPI 和 CL IgG 和 IgM 同种型抗体的存在。与针对 CL 的 APS“标准”抗体相比，APS 患者中存在 AnxA8 IgG 同种型抗体的频率相似（P = 0.764）。
我们证明了 AnxA8 IgG 同种型抗体是 APS 诊断的潜在生物标志物。
