Randall Matthew J, Jüngel Astrid, Rimann Markus, Wuertz-Kozak Karin
Department of Health Science and Technology, Institute for Biomechanics, ETH Zürich, Zurich, Switzerland.
Center of Experimental Rheumatology, University Clinic of Rheumatology, Balgrist University Hospital, University Hospital Zurich, Zurich, Switzerland.
Front Bioeng Biotechnol. 2018 Oct 31;6:154. doi: 10.3389/fbioe.2018.00154. eCollection 2018.
The relevance for three-dimensional (3D) tissue culture of skin has been present for almost a century. From using skin biopsies in organ culture, to vascularized organotypic full-thickness reconstructed human skin equivalents, tissue regeneration of 3D skin has reached a golden era. However, the reconstruction of 3D skin still has room to grow and develop. The need for reproducible methodology, physiological structures and tissue architecture, and perfusable vasculature are only recently becoming a reality, though the addition of more complex structures such as glands and tactile corpuscles require advanced technologies. In this review, we will discuss the current methodology for biofabrication of 3D skin models and highlight the advantages and disadvantages of the existing systems as well as emphasize how new techniques can aid in the production of a truly physiologically relevant skin construct for preclinical innovation.
三维（3D）皮肤组织培养的相关性已存在近一个世纪。从在器官培养中使用皮肤活检，到血管化的器官型全层重建人皮肤等效物，3D皮肤的组织再生已进入黄金时代。然而，3D皮肤的重建仍有发展空间。可重复的方法、生理结构和组织结构以及可灌注血管系统的需求直到最近才成为现实，不过添加更复杂的结构（如腺体和触觉小体）需要先进技术。在这篇综述中，我们将讨论当前3D皮肤模型生物制造的方法，突出现有系统的优缺点，并强调新技术如何有助于生产出真正具有生理相关性的皮肤构建体用于临床前创新。
