National Influenza Center, National Center for Communicable Diseases, Ministry of Health, Ulaanbaatar, Mongolia.
School of Bio-Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia.
PLoS One. 2018 Nov 15;13(11):e0206987. doi: 10.1371/journal.pone.0206987. eCollection 2018.
Influenza B virus-caused illness has recently been considered as an urgent public health problem due to substantial morbidity, mortality and life-threatening medical complications. In this study, we have reported the main characteristics of influenza B virus in Mongolia, including prevalence, lineages, suitability with vaccine strains and drug susceptibility against the virus. 15768 specimens were tested by qPCR for detecting influenza viruses. From positive specimens for influenza B virus, the clinical isolates were isolated using MDCK cells. Sequencing analysis, hemagglutination inhibition assay and Neuraminidase inhibitor (NAI) drug susceptibility testing were performed for the clinical isolates. Influenza B virus was around in 3.46% of the samples in Mongolia, and B/Victoria clade-1A and B/Yamagata clade-3 lineages were predominant. Importantly, it was confirmed that the lineages corresponded to the vaccine strains. Moreover, drug susceptibility tests revealed that some Mongolian clinical isolates showed reduced susceptibility to antiviral agents. Interestingly, G104R was identified as a novel mutation, which might have a significant role in drug resistance of the virus. These results describe the characteristics of influenza B viruses that have caused respiratory illness in the population of Mongolia between 2013 and 2017.
乙型流感病毒引起的疾病由于发病率高、死亡率高以及危及生命的医疗并发症而被认为是当前一个紧迫的公共卫生问题。在本研究中，我们报告了蒙古乙型流感病毒的主要特征，包括流行率、谱系、与疫苗株的匹配性以及对病毒的药物敏感性。使用 qPCR 对 15768 个标本进行流感病毒检测。从乙型流感病毒阳性标本中，使用 MDCK 细胞分离临床分离株。对临床分离株进行测序分析、血凝抑制试验和神经氨酸酶抑制剂（NAI）药物敏感性测试。乙型流感病毒在蒙古的样本中占 3.46%，B/Victoria 谱系 1A 和 B/Yamagata 谱系 3 是主要的谱系。重要的是，证实了这些谱系与疫苗株相对应。此外，药物敏感性测试显示，一些蒙古临床分离株对抗病毒药物的敏感性降低。有趣的是，鉴定出 G104R 是一种新的突变，它可能在病毒的耐药性中起重要作用。这些结果描述了 2013 年至 2017 年期间引起蒙古人群呼吸道疾病的乙型流感病毒的特征。
