Achs M J, Garfinkel D
Department of Computer Science, University of Pennsylvania, Philadelphia 19104.
Am J Physiol. 1988 Aug;255(2 Pt 1):E201-5. doi: 10.1152/ajpendo.1988.255.2.E201.
We have previously modeled pancreatic islet glycolysis under idealized steady-state conditions where the input is a pure hexose anomer and there is no mutarotation and reproduced the known preference for the alpha-anomers of glucose and mannose as substrates. This model is here extended to simulate real experiments, where the hexoses mutarotate and measurements may be taken over time. The behavior of our model system agrees with available experimental data. The hexose diphosphate activators of phosphofructokinase, whose effect was seen as not important in the preceding steady-state analysis, are found here to have a modest (approximately 10-15%) effect on its flux. The previous conclusion that the anomeric preference of the glycolytic pathway follows from that of glucokinase continues to hold in the real experimental situation.
我们之前在理想化的稳态条件下对胰岛糖酵解进行了建模，其中输入为纯己糖异头物，不存在变旋现象，并重现了已知的对葡萄糖和甘露糖α-异头物作为底物的偏好。在此，该模型得到扩展以模拟实际实验，即己糖发生变旋且测量可随时间进行。我们模型系统的行为与现有实验数据相符。磷酸果糖激酶的己糖二磷酸激活剂，其在之前的稳态分析中被认为作用不大，在此发现对其通量有适度（约10 - 15%）的影响。糖酵解途径的异头物偏好源于葡萄糖激酶的异头物偏好这一先前结论在实际实验情况下仍然成立。
