Ahn Seonjoo, Jun Sangmi, Ro Hyun-Joo, Kim Ju Han, Kim Seil
Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Republic of Korea.
Division of Chemical and Medical Metrology, Center for Bioanalysis, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol. 2018 Oct 28;28(10):1760-1768. doi: 10.4014/jmb.1712.12006.
The type strain subsp. KCTC 3135 was deeply sequenced and annotated, replacing a previous draft genome in this study. The and genes were involved in synthesizing wall teichoic acids (WTAs), and these genes and their products were previously regarded as the distinguishing difference between and . However, a comparative genomic analysis of spp. revealed that both and had various types of cell walls. These and operons were mutually exclusive and the genes from were very similar to the genes from non- bacteria, unlike the genes from . The results and previous studies suggest that the genes and the genes are not inherited after subspecies speciation. The phylogenetic tree based on whole genome sequences showed that each subspecies clearly formed a monophyletic group, while the tree based on genes showed that monophyletic groups were formed according to the cell wall type rather than the subspecies. These findings indicate that the genes and the presence of ribitol as a cell-wall constituent were not the distinguishing difference between the subspecies of and that the description of subspecies should be updated.
对模式菌株亚种KCTC 3135进行了深度测序和注释，取代了本研究中之前的基因组草图。和基因参与壁磷壁酸（WTAs）的合成，这些基因及其产物以前被认为是和之间的显著差异。然而，对 spp. 的比较基因组分析表明，和都有多种类型的细胞壁。这些和操纵子相互排斥，来自的基因与来自非细菌的基因非常相似，这与来自的基因不同。结果和先前的研究表明，基因和基因在亚种形成后并未遗传。基于全基因组序列的系统发育树表明，每个亚种都明显形成一个单系群，而基于基因的树表明单系群是根据细胞壁类型而非亚种形成的。这些发现表明，基因和作为细胞壁成分的核糖醇的存在并不是和亚种之间的显著差异，亚种的描述应该更新。
