Wilton Emily E, Opyr Michael P, Kailasam Senthilkumar, Kothe Ronja F, Wieden Hans-Joachim
Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry , University of Lethbridge , Lethbridge , Alberta T1K 3M4 , Canada.
NMR Facility , University of Lethbridge , Lethbridge , Alberta T1K 3M4 , Canada.
ACS Synth Biol. 2018 Nov 16;7(11):2480-2484. doi: 10.1021/acssynbio.8b00407.
The Single Domain Antibody Database, or sdAb-DB, ( www.sdab-db.ca ) is the first freely available repository for single domain antibodies and related classes of proteins. Due to their small size, modular structure, and ease of expression, single domain antibodies (sdAb) have a wide range of applications, including as a rational design tool, and are therefore of great interest for synthetic biologists and bioengineers. However, to enable effective use and sharing of existing sdAbs, including those with engineered functions ( e.g., fusions with fluorescent proteins), as well as the rational design and engineering of new sdAbs, it is necessary to have access to sequences and experimental data. We have therefore developed a publicly available, sdAb-focused database, providing access to manually curated sdAb data from protein databases, published scientific articles, and user submissions. The sdAb-DB is an open-source repository and sharing platform for the sdAb community, providing access to performance data and basic bioinformatic tools for use with previously described and validated sdAbs, as well as for the engineering of new sdAb-based designs and proteins.
单域抗体数据库（Single Domain Antibody Database，简称sdAb-DB，网址为www.sdab-db.ca）是首个免费提供单域抗体及相关蛋白类别的储存库。由于单域抗体（sdAb）体积小、结构模块化且易于表达，因此具有广泛的应用，包括作为合理设计工具，故而受到合成生物学家和生物工程师的极大关注。然而，为了能有效利用和共享现有的单域抗体，包括那些具有工程化功能的抗体（例如与荧光蛋白的融合体），以及合理设计和构建新的单域抗体，获取序列和实验数据是必要的。因此，我们开发了一个公开可用的、以单域抗体为重点的数据库，提供从蛋白质数据库、已发表的科学文章以及用户提交内容中手动整理的单域抗体数据。sdAb-DB是一个面向单域抗体社区的开源储存库和共享平台，提供性能数据和基本生物信息学工具，用于已描述和验证的单域抗体，以及用于构建基于新单域抗体的设计和蛋白质。
