II型胶原提取物对甲氨蝶呤诱导的大鼠免疫抑制的影响。

Effect of type II collagen extract on immunosuppression induced by methotrexate in rats.

作者信息

Kim Ee-Hwa, Kim Yong-Min, Suh Jung-Ho

机构信息

Deptartment of Meridian and Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea.

Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University, Jecheon, Korea.

出版信息

J Exerc Rehabil. 2018 Oct 31;14(5):731-738. doi: 10.12965/jer.1836480.240. eCollection 2018 Oct.

DOI:10.12965/jer.1836480.240

PMID:30443517

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6222161/

Abstract

This study investigated the effect of type II collagen extract on SD rats with deteriorated immunity caused by methotrexate. The test samples were dosed once a day for 28 days by gastric gavage at dosage 250 mg/kg and 500 mg/kg after methotrexate treatment, and the changes on body weight, total blood leukocyte numbers, the percentages of B-cells, CD4+ T-cells and CD8+ T-cells in the blood and spleen were observed. The changes on body weight, the total blood leukocyte numbers, the total lymphocyte numbers in the spleen, the ratio of CD4+ and CD8+ T-cells in the blood and spleen were increased significantly in type II collagen extract groups as compared with the control group. According to the above results, type II collagen extract has an effect of increasing immune responses on rats with deteriorated immunity caused by methotrexate.

摘要

本研究探讨了II型胶原提取物对甲氨蝶呤所致免疫功能低下SD大鼠的影响。甲氨蝶呤处理后，将受试样品按250mg/kg和500mg/kg剂量灌胃给药，每日1次，共28天，观察大鼠体重、外周血白细胞总数、血液及脾脏中B细胞、CD4+T细胞和CD8+T细胞百分比的变化。与对照组相比，II型胶原提取物各剂量组大鼠体重、外周血白细胞总数、脾脏中淋巴细胞总数、血液及脾脏中CD4+与CD8+T细胞比值均显著升高。根据上述结果，II型胶原提取物对甲氨蝶呤所致免疫功能低下大鼠具有增强免疫反应的作用。