Novel Devices Laboratory, College of Engineering, University of Cincinnati, Cincinnati, Ohio 45221, USA.
Lab Chip. 2018 Dec 4;18(24):3750-3759. doi: 10.1039/c8lc01082j.
A wearable sweat biosensing device is demonstrated that stimulates sweat and continuously measures sweat ethanol concentrations at 25 s intervals, which is then correlated with blood ethanol during a >3 hour testing phase. The testing involves a baseline condition (no ethanol) followed by a rapid blood and sweat rise of ethanol (oral bolus), and finally, the physiological response of the body as ethanol concentrations return to baseline (metabolized). Data sets include multiple in vivo validation trials and careful in vitro characterization of the electrochemical enzymatic ethanol sensor against likely interferents. Furthermore, the data is analyzed through known pharmacokinetic models with a strong linear Pearson correlation of 0.9474-0.9996. The continuous nature of the data also allows analysis of blood-to-sweat lag times that range between 2.3 to 11.41 min for ethanol signal onset and 19.32 to 34.44 min for the overall pharmacokinetic curve lag time. This work represents a significant advance that builds upon a continuum of previous work. However, unresolved questions include operation for 24 hours or greater and with analytes beyond those commonly explored for sweat (electrolytes and metabolites). Regardless, this work validates that sweat biosensing can provide continuous and blood-correlated data in an integrated wearable device.
一种可穿戴汗液生物传感设备被展示出来，它可以刺激汗液分泌，并以 25 秒的间隔连续测量汗液中的乙醇浓度，然后在超过 3 小时的测试阶段将其与血液中的乙醇浓度相关联。测试包括基线条件（无乙醇），随后是乙醇的快速血液和汗液上升（口服推注），最后是乙醇浓度回到基线时身体的生理反应（代谢）。数据集包括多个体内验证试验和对电化学酶乙醇传感器对可能的干扰物的仔细体外特征描述。此外，通过已知的药代动力学模型对数据进行分析，得到了 0.9474-0.9996 的强线性 Pearson 相关性。数据的连续性还允许分析血液到汗液的滞后时间，对于乙醇信号的起始，滞后时间范围在 2.3 到 11.41 分钟之间，对于整个药代动力学曲线的滞后时间，范围在 19.32 到 34.44 分钟之间。这项工作是在前人工作的基础上取得的重大进展。然而，仍存在一些未解决的问题，包括 24 小时或更长时间的运行时间，以及除了汗液中常见的电解质和代谢物之外的其他分析物。无论如何，这项工作验证了汗液生物传感可以在集成的可穿戴设备中提供连续的、与血液相关的数据。
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