Duangupama Thitikorn, Pittayakhajonwut Pattama, Thawai Chitti
1Department of Biology, Faculty of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.
2National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Paholyothin Road, Klong Luang, Pathumthani 12120, Thailand.
Int J Syst Evol Microbiol. 2019 Jan;69(1):134-138. doi: 10.1099/ijsem.0.003115. Epub 2018 Nov 16.
An aerobic, spore-forming, actinomycete, designated strain CHM3-46, was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46 produced short, straight chains of warty spores on aerial mycelia. The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan. The whole-cell reducing sugars were glucose, mannose, galacose and ribose. The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H8). 10-methyl C17 : 0, C16 : 0, C17 : 0 and iso-C16 : 0 were identified as the main cellular fatty acids. The G+C content of the genomic DNA was 73.2 mol%. Analysis of the 16S rRNA gene sequence showed that strain CHM3-46 belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521 (98.5 %). Furthermore, a low DNA relatedness value (23.4 %±0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46 and its closest relative. Based on the taxonomic data, strain CHM3-46 could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellisporasoli sp. nov. is proposed. The type strain is CHM3-46 (=TBRC 7649=NBRC 113148).
从泰国清迈府一个温泉池的土壤中分离出一株需氧、产芽孢的放线菌，命名为CHM3-46菌株。该菌株表现出与热猫孢菌属一致的分类特征。CHM3-46菌株在气生菌丝体上产生短的、直链的有疣孢子。在细胞壁肽聚糖中观察到内消旋二氨基庚二酸的存在。全细胞还原糖为葡萄糖、甘露糖、半乳糖和核糖。磷脂包括磷脂酰甲基乙醇胺、羟基磷脂酰甲基乙醇胺、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰肌醇、四种磷酸糖脂和三种未鉴定的磷脂。主要的甲基萘醌是MK-9(H4)、MK-9(H6)和MK-9(H8)。已鉴定出10-甲基C17 : 0、C16 : 0、C17 : 0和异-C16 : 0为主要的细胞脂肪酸。基因组DNA的G+C含量为73.2mol%。16S rRNA基因序列分析表明，CHM3-46菌株属于热猫孢菌属，与腾冲热猫孢菌YIM 77521的相似性最高(98.5%)。此外，在CHM3-46菌株与其最接近的亲缘菌株之间检测到较低的DNA相关性值(23.4%±0.8)以及一些生理和生化特征差异。基于分类学数据，CHM3-46菌株很容易与其最接近的系统发育亲缘菌株区分开来，代表一个新物种，为此提出了新名称热猫孢菌土壤种（Thermocatellispora soli sp. nov.）。模式菌株为CHM3-46(=TBRC 7649=NBRC 113148)。
