MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry , Fuzhou University , Fuzhou 350116 , People's Republic of China.
Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai Jiao Tong University , Shanghai 200240 , People's Republic of China.
Anal Chem. 2018 Dec 18;90(24):14433-14438. doi: 10.1021/acs.analchem.8b04015. Epub 2018 Nov 29.
On cell-membrane surfaces, receptor-protein dimers play fundamental roles in many signaling pathways that are crucial for normal biological processes and cancer development. Efficient and sensitive analysis of receptor dimers in the native environment is highly desirable. Herein, we present a strategy for amplified imaging of receptor dimers in zebrafish and living cells that relies on aptamer recognition and proximity-induced hybridization chain reaction. Taking advantage of specific aptamer recognition and enzyme-free signal amplification, this strategy is successfully applied to the visualization of c-Met-receptor dimers in an HGF-independent or -dependent manner. Therefore, the developed imaging strategy paves the way for further investigation of the dimerization or oligomerization states of cell-surface receptors and their corresponding activation processes in zebrafish and living cells.
在细胞膜表面，受体蛋白二聚体在许多信号通路中发挥着重要作用，这些信号通路对于正常的生物过程和癌症的发展至关重要。在原生环境中高效、灵敏地分析受体二聚体是非常理想的。在此，我们提出了一种依赖于适体识别和邻近诱导杂交链反应的斑马鱼和活细胞中受体二聚体放大成像的策略。利用特异性适体识别和无酶信号放大，该策略成功地应用于 HGF 非依赖性或依赖性方式下 c-Met 受体二聚体的可视化。因此，所开发的成像策略为进一步研究斑马鱼和活细胞中细胞表面受体的二聚化或寡聚化状态及其相应的激活过程铺平了道路。
