鉴定和调控心力衰竭中长链非编码 RNA Heat2。
Identification and regulation of the long non-coding RNA Heat2 in heart failure.
机构信息
Institute for Cardiovascular Regeneration, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; Department of Cardiology, Internal Medicine III, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany; Clinic and Polyclinic for Cardiology, University Hospital Leipzig, Germany.
Institute for Cardiovascular Regeneration, Goethe-University Hospital, Theodor Stern Kai 7, Frankfurt, Germany.
出版信息
J Mol Cell Cardiol. 2019 Jan;126:13-22. doi: 10.1016/j.yjmcc.2018.11.004. Epub 2018 Nov 13.
AIMS
Circulating immune cells have a significant impact on progression and outcome of heart failure. Long non-coding RNAs (lncRNAs) comprise novel epigenetic regulators which control cardiovascular diseases and inflammatory disorders. We aimed to identify lncRNAs regulated in circulating immune cells of the blood of heart failure patients.
METHODS AND RESULTS
Next-generation sequencing revealed 110 potentially non-coding RNA transcripts differentially expressed in peripheral blood mononuclear cells of heart failure patients with reduced ejection fraction. The up-regulated lncRNA Heat2 was further functionally characterized. Heat2 expression was detected in whole blood, PBMNCs, eosinophil and basophil granulocytes. Heat2 regulates cell division, invasion, transmigration and immune cell adhesion on endothelial cells.
CONCLUSION
Heat2 is an immune cell enriched lncRNA that is elevated in the blood of heart failure patients and controls cellular functions.
目的
循环免疫细胞对心力衰竭的进展和结局有重大影响。长链非编码 RNA（lncRNA）是新型的表观遗传调控因子，可控制心血管疾病和炎症性疾病。我们旨在鉴定心力衰竭患者血液中循环免疫细胞中受调控的 lncRNA。
方法和结果
下一代测序显示，在射血分数降低的心力衰竭患者的外周血单个核细胞中，有 110 种潜在的非编码 RNA 转录本表达差异。进一步对上调的 lncRNA Heat2 进行了功能表征。Heat2 表达可在全血、PBMNCs、嗜酸性粒细胞和嗜碱性粒细胞中检测到。Heat2 调节细胞分裂、侵袭、迁移和免疫细胞在内皮细胞上的黏附。
结论
Heat2 是一种富含免疫细胞的 lncRNA，在心力衰竭患者的血液中升高，并控制细胞功能。
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