Laboratory of Environmental Microbiology, Institute of Microbiology of the CAS, Vídeňská 1083, 14220 Praha 4, Czech Republic.
Helmholtz-Centre for Environmental Research - UFZ, Department of Molecular Systems Biology, Permoserstraße 15, 04318 Leipzig, Germany.
J Proteomics. 2019 Apr 30;198:50-58. doi: 10.1016/j.jprot.2018.11.011. Epub 2018 Nov 13.
Metaproteomics was established to analyse both the structure and the function of microbial communities and, particularly in soils, their contribution to ecosystem services. In this review, we provide an overview on how the study of the soil metaproteome can provide fundamental information on the role of microbial communities in soil ecosystem services. We further discuss the strengths and weaknesses of soil metaproteomics in comparison to other culture-independent OMIC techniques. We critically review its bottlenecks but also provide strategies to mitigate and possible directions for future research as the direct link of structure and function is advantageous and complementary to metagenomics, metatranscriptomics and metametabolomics.
宏蛋白质组学的建立是为了分析微生物群落的结构和功能，特别是在土壤中，它们对生态系统服务的贡献。在这篇综述中，我们概述了研究土壤宏蛋白质组学如何为微生物群落在土壤生态系统服务中的作用提供基础信息。我们进一步讨论了与其他非培养 OMIC 技术相比，土壤宏蛋白质组学的优缺点。我们批判性地审查了它的瓶颈，但也提供了减轻瓶颈的策略和未来研究的可能方向，因为结构和功能的直接联系对宏基因组学、宏转录组学和宏代谢组学是有利和互补的。
