Department of Biological and Food Engineering, Bozhou University, Bozhou, Anhui 236800, China.
Key Laboratory of Industrial Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
FEMS Microbiol Lett. 2018 Dec 1;365(24). doi: 10.1093/femsle/fny263.
Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits and internal transcribed spacers sequences, indicating that endophytic fungi of P. lactiflora are abundant and diverse. The dominant endophytic fungi were Aspergillus, Alternaria and Penicillium. More fungi were recovered from leaves than from roots and stems. The similarity index was highest between the stems and leaves (0.733), followed by the roots and leaves (0.615) and the stems and roots (0.563). Analyses of the fermentation extracts of 22 endophytic fungi by high-performance liquid chromatography and mass spectrometry revealed that three strains (R12, Alternaria tenuissima; S4, Aspergillus flavus; and R17 Penicillium commune) were able to produce paeoniflorin. Among the paeoniflorin-producing fungi, the yield of paeoniflorin from A. flavus S4 was 342.4 μg/L, and this strain could be used as a candidate for the industrial production of paeoniflorin.
从主要分布在中国的牡丹（Paeonia lactiflora Pallas）中分离出内生真菌，并对其进行鉴定，以筛选出能够产生芍药苷的内生真菌。从牡丹的根、茎和叶中获得了 101 个分离物，根据形态特征和内部转录间隔区序列将其分为 16 个真菌分类群，表明牡丹的内生真菌丰富多样。优势内生真菌为曲霉属、链格孢属和青霉属。从叶片中分离出的真菌多于从根和茎中分离出的真菌。茎和叶之间的相似性指数最高（0.733），其次是根和叶（0.615）和茎和根（0.563）。通过高效液相色谱和质谱分析 22 株内生真菌的发酵提取物发现，有 3 株（R12，细极链格孢；S4，黄曲霉；和 R17，普通青霉）能够产生芍药苷。在产芍药苷的真菌中，黄曲霉 S4 的芍药苷产量为 342.4μg/L，该菌株可作为工业生产芍药苷的候选菌株。
