Turani Blerta, Aliko Valbona, Faggio Caterina
Department of Biology, Faculty of Natural Sciences, University of Tirana, Tirana, Albania.
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Nat Prod Res. 2020 Mar;34(6):830-837. doi: 10.1080/14786419.2018.1508147. Epub 2018 Nov 16.
Amphibian egg-jelly coat plays an important role in successful fertilization and development. Here, we ask whether proteins like allurin in the jelly coats of frog eggs might influence fertilization rate success. Using in vitro fertilization of Albanian water frog, , we found that body cavity eggs or eggs deprived of jelly coat were not fertilized, compromising the success of in vitro fertilization procedure. When de-jellied eggs were inseminated with sperm suspension, the fertilization efficiency is dramatically decreased even inhibited, suggesting that the gel structure is one of the major factors in the achievement of fertilization in the frogs. Fertilization of de-jellied eggs with sperm pre-treated with egg jelly coat, restored the fertilization competency. Such a result suggests that egg jelly coat probably guides the sperm to the egg surface while maintaining the fertilization ability, contributing to a successful in vitro fertilization of .
两栖动物的卵胶膜在成功受精和发育过程中起着重要作用。在此，我们探讨蛙卵胶膜中的蛋白质（如allurin）是否可能影响受精成功率。通过阿尔巴尼亚水蛙的体外受精实验，我们发现体腔卵或去除胶膜的卵无法受精，这影响了体外受精程序的成功率。当用精子悬浮液对去胶膜的卵进行授精时，受精效率显著降低甚至受到抑制，这表明凝胶结构是蛙类实现受精的主要因素之一。用经卵胶膜预处理的精子对去胶膜的卵进行受精，可恢复受精能力。这一结果表明，卵胶膜可能在引导精子到达卵表面的同时维持受精能力，有助于阿尔巴尼亚水蛙的体外受精成功。
