Watanabe M, Uchiyama Y
Department of Anatomy, University of Tsukuba, Japan.
Cell Tissue Res. 1988 Aug;253(2):337-45. doi: 10.1007/BF00222290.
Variations in stomach weights, plasma glucose and insulin levels in the portal vein, and in subcellular structures of rat pancreatic islet B-, A- and D-cells were examined over 24 h. The variation in stomach weights paralleled plasma glucose levels, indicating that the levels may be influenced by intestinal glucose absorption. Plasma insulin levels increased from the late dark to the early light periods, whereas they decreased from the late light to the early dark periods. The variation in plasma insulin levels was in the opposite sense to that in the relative numbers of B-cell granules. The decrease in the relative numbers of A-cell granules occurred between the late dark and early light periods. The relative numbers of D-cell granules decreased before and after the decreases in B- and A-cell granules. The variation in D-cell granules appears to correspond to the inhibitory effect of somatostatin. The relative amounts of rough endoplasmic reticulum (rER) in each cell varied in a reciprocal manner to those of B-cell granules. Moreover, the variation in plasma insulin levels coincided with variations in rER of hepatocytes and pancreatic acinar cells. The changes in rER of each cell may correlate with the trophic effect of insulin.
在24小时内检测了大鼠胃重量、门静脉血浆葡萄糖和胰岛素水平以及胰岛B细胞、A细胞和D细胞亚细胞结构的变化。胃重量的变化与血浆葡萄糖水平平行，表明这些水平可能受肠道葡萄糖吸收的影响。血浆胰岛素水平从黑暗后期到光照前期升高，而从光照后期到黑暗前期降低。血浆胰岛素水平的变化与B细胞颗粒相对数量的变化方向相反。A细胞颗粒相对数量的减少发生在黑暗后期和光照前期之间。D细胞颗粒的相对数量在B细胞和A细胞颗粒减少之前和之后均减少。D细胞颗粒的变化似乎与生长抑素的抑制作用相对应。每个细胞中粗面内质网（rER）的相对量与B细胞颗粒的相对量呈相反变化。此外，血浆胰岛素水平的变化与肝细胞和胰腺腺泡细胞rER的变化一致。每个细胞中rER的变化可能与胰岛素的营养作用相关。
