Azimaraghi Omid, Nezhad Sistani Maryam, Abdollahifar Mohammad-Amin, Movafegh Ali, Maleki Anahid, Soltani Ebrahim, Shahbazkhani Alireza, Atef-Yekta Reza
Tehran University of Medical Sciences, Dr. Shariati Hospital, Department of Anesthesiology and Critical Care Medicine, Teerã, Irã.
Shahid Beheshti University of Medical Sciences, Department of Anatomical Sciences, Teerã, Irã.
Braz J Anesthesiol. 2019 Jan-Feb;69(1):58-63. doi: 10.1016/j.bjan.2018.09.001. Epub 2018 Nov 13.
Developing brain is more vulnerable to environmental risk than is the developed brain. We evaluated the effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus using stereological methods.
Eighteen neonatal male mice were randomly divided into three groups. Group A, inhaled sevoflurane at a concentration of 1.5%; Group B, inhaled sevoflurane at a concentration of 3%; and Group C (control group), inhaled only 100% oxygen. Treatments were applied for 30 min a day for 7 consecutive days. The hippocampal volume, dendrite length, number of neurons, and number of glial cells were evaluated in each group using stereological estimations.
We identified a ∼2% reduction in the volume of the hippocampus in Group A compared to Group C. Mean hippocampal volume was ∼11% smaller in Group B than it was in Group C. However, these differences in hippocampal volume between the groups were not statistically significant ( > 0.05 for all). As for the number of neurons, we found significantly fewer neurons in Group A (∼29% less) and Group B (∼43% less) than we did in Group C ( < 0.05 for both). The dendrite length was ∼8% shorter in Group A and ∼11% shorter in Group B than it was in Group C.
Repeated exposure to sevoflurane, regardless of the concentration, reduced the volume of the neonatal mouse hippocampus, as well as the number of neurons and dendrite length.
发育中的大脑比发育成熟的大脑更容易受到环境风险的影响。我们使用体视学方法评估了反复暴露于不同浓度七氟醚对新生小鼠海马体的影响。
18只新生雄性小鼠被随机分为三组。A组吸入浓度为1.5%的七氟醚；B组吸入浓度为3%的七氟醚；C组（对照组）仅吸入100%的氧气。每天治疗30分钟，连续治疗7天。使用体视学估计法评估每组小鼠的海马体体积、树突长度、神经元数量和神经胶质细胞数量。
我们发现，与C组相比，A组海马体体积减少了约2%。B组海马体平均体积比C组小约11%。然而，各组之间海马体体积的这些差异无统计学意义（所有P>0.05）。至于神经元数量，我们发现A组（减少约29%）和B组（减少约43%）的神经元数量明显少于C组（两组P<0.05）。A组的树突长度比C组短约8%，B组比C组短约11%。
反复暴露于七氟醚，无论浓度如何，都会减少新生小鼠海马体的体积，以及神经元数量和树突长度。
