Laboratory of Pain Pharmacology and Neuromodulation: Pre clinical studies, Pharmacology Department, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil; Post-Graduate Program in Medicine: Medical Sciences, School of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Post-Graduate Program in Biological Sciences, Pharmacology and Therapeutics, Institute of Basic Health Sciences, Universidade Federal Rio Grande do Sul, Porto Alegre, Brazil; Laboratory of Pain Pharmacology and Neuromodulation: Pre clinical studies, Pharmacology Department, Institute of Basic Health Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil; Animal Experimentation Unit and Graduate Research Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-003, Brazil.
Neuropeptides. 2019 Feb;73:1-10. doi: 10.1016/j.npep.2018.09.006. Epub 2018 Oct 31.
Obesity is a multifactorial disease associated with metabolic dysfunction and the prevention and treatment of obesity are often unsatisfactory. Transcranial direct-current stimulation (tDCS) is a non-invasive brain stimulation technique that has proven promising in the treatment of eating disorders such as obesity. We investigate the effects of tDCS on locomotor and exploratory activities, anxiety-like and feeding behavior, and levels of brain-derived neurotrophic factor (BDNF), IL (interleukin)-10, IL-1β, and tumor necrosis factor-alpha (TNF-α) in the cerebral cortex of obese rats. A total of 40 adult male Wistar rats were used in our study. Animals were divided into groups of three or four animals per cage and allocated to four treatment groups: standard diet plus sham tDCS treatment (SDS), standard diet plus tDCS treatment (SDT), hypercaloric diet plus sham tDCS treatment (HDS), hypercaloric diet plus tDCS treatment (HDT). After 40 days on a hypercaloric diet and/or standard diet were to assessed the locomotor and exploratory activity and anxiety-like behavior to by the open field (OF) and elevated plus maze (EPM) tests respectively before and after exposure to tDCS treatment. The experimental groups were submitted to active or sham treatment tDCS during eight days. Palatable food consumption test (PFT) was performed 24 h after the last tDCS session under fasting and feeding conditions. Obese animals submitted to tDCS treatment showed a reduction in the Lee index, visceral adipose tissue weight, and food craving. In addition, bicephalic tDCS decreased the cerebral cortex levels of IL-1β and TNF-α in these animals. Exposure to a hypercaloric diet produced an anxiolytic effect, which was reversed by bicephalic tDCS treatment. These results suggest that, in accordance with studies in humans, bicephalic tDCS could modulate biometric and inflammatory parameters, as well as anxiety-like and feeding behavior, of rats subjected to the consumption of a hypercaloric diet.
肥胖是一种与代谢功能障碍相关的多因素疾病，肥胖的预防和治疗往往不尽如人意。经颅直流电刺激（tDCS）是一种非侵入性的脑刺激技术，已被证明在治疗肥胖等饮食失调方面有很大的潜力。我们研究了 tDCS 对肥胖大鼠大脑皮质运动和探索活动、焦虑样行为和摄食行为以及脑源性神经营养因子（BDNF）、白细胞介素（IL）-10、IL-1β和肿瘤坏死因子-α（TNF-α）水平的影响。本研究共使用了 40 只成年雄性 Wistar 大鼠。将动物分为每笼 3 或 4 只动物的组，并分为 4 个治疗组：标准饮食加假 tDCS 治疗（SDS）、标准饮食加 tDCS 治疗（SDT）、高热量饮食加假 tDCS 治疗（HDS）、高热量饮食加 tDCS 治疗（HDT）。在高热量饮食和/或标准饮食 40 天后，通过旷场（OF）和高架十字迷宫（EPM）测试分别评估运动和探索活动以及焦虑样行为，然后再进行 tDCS 治疗。实验组在 8 天内接受主动或假 tDCS 治疗。在最后一次 tDCS 治疗后 24 小时禁食和进食条件下进行美味食物消耗测试（PFT）。接受 tDCS 治疗的肥胖动物的 Lee 指数、内脏脂肪组织重量和食物渴望均有所降低。此外，双极 tDCS 降低了这些动物大脑皮质中 IL-1β和 TNF-α的水平。暴露于高热量饮食产生了抗焦虑作用，而双极 tDCS 治疗则逆转了这种作用。这些结果表明，与人类研究一致，双极 tDCS 可以调节摄入高热量饮食的大鼠的生物计量和炎症参数，以及焦虑样行为和摄食行为。
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