CSIR Institute of Genomics and Integrative Biology (IGIB), Mathura Road, New Delhi 110025, India.
Academy of Scientific and Innovative Research (AcSIR), CSIR Human Resource Development Centre (CSIR-HRDC), Campus Sector 19, Kamla Nehru Nagar, Ghaziabad, Uttar Pradesh 201 002, India.
J Exp Biol. 2019 Jan 10;222(Pt 1):jeb192195. doi: 10.1242/jeb.192195.
High fecundity, transparent embryos for monitoring the rapid development of organs and the availability of a well-annotated genome has made zebrafish a model organism of choice for developmental biology and neurobiology. This vertebrate model, which is also a favourite in chronobiology studies, shows striking circadian rhythmicity in behaviour. Here, we identify novel genes in the zebrafish genome that are expressed in the zebrafish retina. We further resolve the expression pattern over time and tentatively assign specific novel transcripts to retinal bipolar cells of the inner nuclear layer. Using chemical ablation and free run experiments, we segregate the transcripts that are rhythmic when entrained by light from those that show sustained oscillations in the absence of external cues. The transcripts reported here with rigorous annotation and specific functions in circadian biology provide the groundwork for functional characterization of novel players in the zebrafish retinal clock.
高繁殖力、透明胚胎可用于监测器官的快速发育，以及注释良好的基因组，这些使斑马鱼成为发育生物学和神经生物学的首选模式生物。这种脊椎动物模型在生物钟生物学研究中也很受欢迎，其行为表现出显著的昼夜节律性。在这里，我们在斑马鱼基因组中鉴定出在斑马鱼视网膜中表达的新基因。我们进一步解析了随时间的表达模式，并初步将特定的新转录本分配给内核层的视网膜双极细胞。通过化学消融和自由运行实验，我们将那些被光照同步的转录本与那些在没有外部线索的情况下持续振荡的转录本区分开来。这里报道的具有严格注释和在生物钟生物学中特定功能的转录本为功能表征斑马鱼视网膜时钟中的新型调控因子奠定了基础。
