Eslami Mohsen, Zadeh Hashem Elham, Ghaniei Abolfazl, Sayyah-Atashbeig Hossein
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Cell Tissue Bank. 2018 Dec;19(4):799-807. doi: 10.1007/s10561-018-9738-6. Epub 2018 Nov 16.
Sustainable reduction in semen quality due to detrimental effects of primary and secondary peroxidative products was occurred during liquid storage. The objective of the current experiment was to explore the influence of bovine serum albumin conjugated linoleic acid (LA) on the rooster spermatozoa routine tests and lipid peroxidative/antioxidative levels stored at 4 °C over 48 h. For this purpose, collected ejaculates (≥ 80% progressive motile spermatozoa) pooled and extended with the phosphate buffer medium without (control) or enriched with different amounts of LA (0.125, 0.25 or 0.50 mM). Contents of total antioxidant status (TAS) and thiobarbituric reactive substances (TBARS) were measured separately in the medium and spermatozoa, as well as percent of viability and motility at 0, 24 and 48 h intervals. Viability was not affected by treatment during the study intervals (P > 0.05). While, higher motility was recorded in LA 0.50 mM group (77.98 ± 1.89 and 57.02 ± 2.45) compared to the control group (68.78 ± 1.29 and 45.09 ± 1.86) at 24 and 48 h, respectively (P < 0.03). Amounts of TBARS in medium and spermatozoa were lower in LA 0.25 and 0.50 groups compared to the control at 48 h (P < 0.01). Moreover, TAS levels of medium and spermatozoa were lower in control samples compared to LA treated groups at 48 h (P < 0.03). Because of the ability of the LA to lowering the quantities of lipid peroxidation index and improving motility especially at 0.5 mM levels, it can be proposed as an additive during liquid storage of rooster semen.
由于一级和二级过氧化产物的有害影响，精液质量在液体保存期间持续下降。本实验的目的是探讨牛血清白蛋白共轭亚油酸（LA）对公鸡精子在4℃下保存48小时的常规检测以及脂质过氧化/抗氧化水平的影响。为此，收集的射精样本（≥80%进行性运动精子）混合后，用不含（对照组）或添加不同量LA（0.125、0.25或0.50 mM）的磷酸盐缓冲液培养基进行稀释。分别在培养基和精子中测量总抗氧化状态（TAS）和硫代巴比妥酸反应物质（TBARS）的含量，以及在0、24和48小时间隔时的活力和运动率百分比。在研究期间，活力不受处理的影响（P>0.05）。然而，与对照组相比，在24小时和48小时时，0.50 mM LA组的运动率更高（分别为77.98±1.89和57.02±2.45），而对照组分别为68.78±1.29和45.09±1.86（P<0.03）。在48小时时，0.25和0.50 mM LA组培养基和精子中的TBARS含量低于对照组（P<0.01）。此外，在48小时时，对照组样本中培养基和精子的TAS水平低于LA处理组（P<0.03）。由于LA能够降低脂质过氧化指数的量并提高运动率，尤其是在0.5 mM水平，因此可以建议将其作为公鸡精液液体保存期间的添加剂。
