Mishra Bina, Mondal Piyali, Patel C L, Zafir Insha, Gangwar Rachna, Singh Neha, Sonowal Joyshikh, Bisht Deepanker, Sahu Amit Ranjan, Baig Mumtaz, Sajjanar Basavaraj, Singh R K, Gandham Ravi Kumar
Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India.
Virus Genes. 2019 Feb;55(1):51-59. doi: 10.1007/s11262-018-1613-9. Epub 2018 Nov 16.
Sheeppox disease is associated with significant losses in sheep production world over. The sheep pox virus, the goatpox virus, and the lumpy skin disease virus cannot be distinguished by conventional serological tests. Identification of these pathogens needs molecular methods. In this study, seven genes viz. EEV maturation protein-F12L, Virion protein-D3R, RNA polymerase subunit-A5R, Virion core protein-A10L, EEV glycoprotein-A33R, VARV B22R homologue, and Kelch like protein-A55R that cover the start, middle, and end of the genome were selected. These genes were amplified from Roumanian-Fanar vaccine strain and Jaipur virulent strain, cloned, and sequenced. On analysis with the available database sequences, VARV B22R homologue was identified as a marker for phylogenetic reconstruction for classifying the sheeppox viruses of the ungulates. Further, divergence time dating with VARV B22R gene accurately predicted the sheeppox disease outbreak involving Jaipur virulent strain.
绵羊痘病在全球绵羊生产中会造成重大损失。绵羊痘病毒、山羊痘病毒和结节性皮炎病毒无法通过传统血清学检测加以区分。这些病原体的鉴定需要分子方法。在本研究中，选择了覆盖基因组起始、中间和末端的七个基因，即胞外病毒成熟蛋白-F12L、病毒体蛋白-D3R、RNA聚合酶亚基-A5R、病毒体核心蛋白-A10L、胞外病毒糖蛋白-A33R、痘苗病毒B22R同源物和类Kelch蛋白-A55R。这些基因从罗马尼亚-法纳尔疫苗株和斋浦尔强毒株中扩增、克隆并测序。通过与现有数据库序列进行分析，痘苗病毒B22R同源物被鉴定为用于对有蹄类动物的绵羊痘病毒进行系统发育重建的标记。此外，利用痘苗病毒B22R基因进行分歧时间测定准确预测了涉及斋浦尔强毒株的绵羊痘病疫情。
