Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China.
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, China.
J Biophotonics. 2019 Apr;12(4):e201800327. doi: 10.1002/jbio.201800327. Epub 2018 Dec 26.
Modified nucleoside in urine samples is one of the most common biomarkers for cancer screening. Therefore, we developed a novel detection method for modified nucleoside detection in human urine. In this work, the modified nucleoside from real cancer patient's urine samples was first separated and purified using the affinity chromatography (AC) technology relying on its specific adsorption capacity. Then, surface-enhanced Raman spectroscopy (SERS) technology with the capability of single molecular detection was used to sensitively characterize the biomolecular features of modified nucleoside. A total of 141 high-quality SERS spectra of urinary modified nucleoside can be obtained from 50 gastric cancer patients and 43 breast cancer patients, as well as 48 healthy volunteers. Using principal component analysis combined with linear discriminant analysis (PCA-LDA), the diagnostic sensitivities for identifying gastric cancer vs normal, breast cancer vs normal, gastric cancer vs breast cancer were 84.0%, 76.7% and 82.0%, respectively, and the corresponding diagnostic specificities for each combination were 95.8%, 87.5% and 90.7%, respectively. These results show that this novel method based on urinary modified nucleoside detection combining AC and SERS technologies holds promising potential for developing a specific, non-invasive and label-free tool for cancer screening.
尿液样本中的修饰核苷是癌症筛查中最常用的生物标志物之一。因此，我们开发了一种用于检测人尿液中修饰核苷的新型检测方法。在这项工作中，首先利用基于特定吸附能力的亲和层析（AC）技术分离和纯化来自真实癌症患者尿液样本中的修饰核苷。然后，利用具有单分子检测能力的表面增强拉曼光谱（SERS）技术来灵敏地表征修饰核苷的生物分子特征。从 50 名胃癌患者、43 名乳腺癌患者和 48 名健康志愿者的尿液中总共可以获得 141 个高质量的修饰核苷 SERS 光谱。通过主成分分析结合线性判别分析（PCA-LDA），用于识别胃癌与正常、乳腺癌与正常、胃癌与乳腺癌的诊断灵敏度分别为 84.0%、76.7%和 82.0%，而每种组合的相应诊断特异性分别为 95.8%、87.5%和 90.7%。这些结果表明，这种基于尿液修饰核苷检测的新型方法结合 AC 和 SERS 技术，具有开发用于癌症筛查的特异性、非侵入性和无标记工具的巨大潜力。
