三氯生对原代大鼠颗粒细胞甾体生成的影响及可能机制。
The effects and possible mechanisms of triclosan on steroidogenesis in primary rat granulosa cells.
机构信息
Department of Developmental and Behavioral Pediatrics, Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Ministry of Education Shanghai Key Laboratory of Children's Environmental Health, Shanghai, China.
Department of Developmental and Behavioral Pediatrics, Pediatric Translational Medicine Institute, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Ministry of Education Shanghai Key Laboratory of Children's Environmental Health, Shanghai, China.
出版信息
Reprod Toxicol. 2019 Jan;83:28-37. doi: 10.1016/j.reprotox.2018.11.001. Epub 2018 Nov 14.
BACKGROUND
Triclosan (TCS) has been detected in human tissues. It can disrupt steroidogenesis in vivo. The study on the effects of TCS on ovarian granulosa cells was lacking.
METHODS
Primary rat granulosa cells (rGCs) were treated with TCS. Concentrations of estradiol (E), progesterone (P) in the cell culture supernatants were measured. Microarray was used to measure gene expression profiles. Pathway analysis was performed to identify signaling networks that linked differentially expressed genes (DEGs). Genes related with steroidogenesis were analyzed.
RESULTS
TCS increased E and P production. A total of 2006 DEGs were identified. Pathway analysis revealed that ovarian steroidogenesis pathway was upregulated. Both PCR and Western-blot demonstrated that the expressions of key genes involved in this pathway were significantly increased.
CONCLUSIONS
TCS co-administered with follicle-stimulating hormone (FSH) could increase E and P production in rGCs and up-regulate ovarian steroidogenesis pathway. StAR and aromatase protein were increased by TCS, while P450scc protein wasn't changed significantly.
背景
三氯生（TCS）已在人体组织中被检测到。它可以在体内干扰类固醇的生成。关于 TCS 对卵巢颗粒细胞影响的研究还比较缺乏。
方法
用 TCS 处理原代大鼠颗粒细胞（rGCs）。测量细胞培养上清液中雌二醇（E）和孕酮（P）的浓度。使用微阵列来测量基因表达谱。进行通路分析以确定将差异表达基因（DEGs）联系起来的信号网络。分析与类固醇生成相关的基因。
结果
TCS 增加了 E 和 P 的产生。共鉴定出 2006 个 DEGs。通路分析显示卵巢类固醇生成途径被上调。PCR 和 Western-blot 均表明该途径关键基因的表达显著增加。
结论
TCS 与促卵泡激素（FSH）联合使用可增加 rGCs 中 E 和 P 的产生，并上调卵巢类固醇生成途径。TCS 增加了 StAR 和细胞色素 P450scc 蛋白，而 P450scc 蛋白的含量没有明显变化。
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