Department of Bioinformatics, School of Basic Medical, Southern Medical University, Guangzhou, Guangdong 510515, China; Center for Genome Analysis, ABLife Inc., Optics Valley International Biomedical Park, Building 18-1, East Lake High-Tech Development Zone, 388 Gaoxin 2nd Road, Wuhan, Hubei 430075, China.
Center for Genome Analysis, ABLife Inc., Optics Valley International Biomedical Park, Building 18-1, East Lake High-Tech Development Zone, 388 Gaoxin 2nd Road, Wuhan, Hubei 430075, China; Laboratory for Genome Regulation and Human Health, ABLife Inc., Optics Valley International Biomedical Park, Building 18-2, East Lake High-Tech Development Zone, 388 Gaoxin 2nd Road, Wuhan, Hubei 430075, China.
Gene. 2019 Mar 1;687:125-134. doi: 10.1016/j.gene.2018.11.026. Epub 2018 Nov 14.
RNA binding proteins (RBPs) are key players of genome regulation. Here we report the transcriptome study of HnRNP D-Like protein, which belongs to the hnRNP family. We used RNA-seq to analyze the global transcript level and alternative splicing on hnRNPDL shRNA-treated cells and control. Sh-hnRNPDL extensively increased in the expression of genes involved in female pregnancy, cell apoptosis, cell proliferation and cell migration. HnRNPDL regulated alternative splicing of hundreds of genes enriched in transcription regulation and signaling pathways including NOD-like receptor signaling, Notch signaling, and TNF signaling. This study provides the first transcriptome-wide analysis of hnRNPDL regulation of gene expression, which adds to the understanding of critical hnRNPDL functions.
RNA 结合蛋白（RBPs）是基因组调控的关键参与者。在这里，我们报告了属于 hnRNP 家族的 HnRNP D-Like 蛋白的转录组研究。我们使用 RNA-seq 分析了 hnRNPDL shRNA 处理细胞和对照细胞的全局转录水平和选择性剪接。Sh-hnRNPDL 广泛增加了参与女性妊娠、细胞凋亡、细胞增殖和细胞迁移的基因的表达。HnRNPDL 调节了数百个基因的选择性剪接，这些基因富集在转录调控和信号通路中，包括 NOD 样受体信号、Notch 信号和 TNF 信号。本研究提供了 hnRNPDL 对基因表达调控的首次全转录组分析，这增加了对 hnRNPDL 关键功能的理解。
