College of Animal Science, Zhejiang University, Hangzhou 310058, China.
College of Animal Science, Zhejiang University, Hangzhou 310058, China.
Virology. 2019 Jan 15;527:12-20. doi: 10.1016/j.virol.2018.10.026. Epub 2018 Nov 14.
Bombyx mori nucleopolyhedrovirus (BmNPV) orf11 (bm11) is a highly conserved gene with unknown function. It is homologous to AcMNPV orf19. In this study, a bm11 knockout virus was constructed and its role was investigated. Expression analysis indicated that bm11 is a late gene and confocal microscopy analysis demonstrated that Bm11 localizes predominantly in the nuclear ring zone at the late phase of infection. The bm11 deletion did not affect budded virus (BV) production or viral genome replication, but markedly reduced the production of occlusion bodies (OBs) and the embedding of occlusion-derived viruses (ODVs). Bio-assays showed that Bm11 was involved in BmNPV infectivity in vivo by direct injection. In conclusion, our results demonstrated that although Bm11 is not essential for BV production or mature ODV formation, it affects OB production and ODV occlusion.
家蚕核型多角体病毒（BmNPV）orf11（bm11）是一个具有未知功能的高度保守基因。它与 AcMNPV orf19 同源。在本研究中，构建了一个 bm11 敲除病毒，并研究了其作用。表达分析表明，bm11 是一个晚期基因，共聚焦显微镜分析表明 Bm11 在感染后期主要定位于核环区。bm11 缺失不影响芽殖病毒（BV）的产生或病毒基因组的复制，但显著降低了包埋体（OB）的产生和包埋的出芽型病毒（ODV）。生物测定表明，Bm11 通过直接注射参与 BmNPV 在体内的感染性。总之，我们的结果表明，尽管 Bm11 对于 BV 的产生或成熟的 ODV 形成不是必需的，但它影响 OB 的产生和 ODV 的包埋。
