College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China.
Fish Shellfish Immunol. 2019 Mar;86:46-52. doi: 10.1016/j.fsi.2018.11.031. Epub 2018 Nov 14.
Snakehead vesiculovirus (SHVV) has caused great economic loss in snakehead fish culture in China. However, there is no effective strategy to prevent the epidemic of the virus. Understanding the host factors in response to virus infection is the basis for the prevention of viral disease. In this study, the transcriptomic profiles of SHVV-infected and mock-infected SSN-1 cells (derived from striped snakehead, Channa striatus) at 3 and 24 h (h) post of infection (poi) were obtained using high-throughput sequencing technique. A total of 93,372 unigenes were obtained. The differently expressed genes (DEGs) of SSN-1 cells upon SHVV infection were thereby identified, including 3668 and 3536 DEGs at 3 and 24 h poi, respectively. These DEGs were involved in many pathways of viral pathogenesis, including retinoic acid-inducible gene I (RIG-I) like receptors pathway, Toll-like receptor signaling pathway, NF-kappa B signaling pathway, PI3K-Akt signaling pathway and MAPK signaling pathway. Therefore, several immune-related DEGs were randomly selected and confirmed by quantitative real-time PCR (qRT-PCR). In addition, the effects of the interferon inducible protein 35 (IFI35) on SHVV replication were further investigated. Over-expression or inhibition of IFI35 significantly promoted or reduced SHVV replication at the level of viral gene expression, which indicated that IFI35 might be a positive factor for SHVV replication in SSN-1 cells. Our findings presented some valuable information, which will benefit for future study on SHVV-host interactions.
噬菌头鱼病毒 (SHVV) 在中国的乌鳢养殖中造成了巨大的经济损失。然而，目前尚无有效的策略来预防该病毒的流行。了解宿主对病毒感染的反应的相关因素是预防病毒性疾病的基础。在本研究中，采用高通量测序技术获得了 SHVV 感染和 mock 感染的 SSN-1 细胞（源自条纹乌鳢，Channa striatus）在感染后 3 和 24 小时（h）的转录组图谱。共获得 93372 条 unigenes。因此，鉴定了 SSN-1 细胞在 SHVV 感染时的差异表达基因（DEGs），分别在 3 和 24 h poi 时鉴定出 3668 和 3536 个 DEGs。这些 DEGs 参与了病毒发病机制的许多途径，包括视黄酸诱导基因 I (RIG-I) 样受体途径、Toll 样受体信号通路、NF-kappa B 信号通路、PI3K-Akt 信号通路和 MAPK 信号通路。因此，随机选择了几个免疫相关的 DEGs，并通过定量实时 PCR (qRT-PCR) 进行了验证。此外，还进一步研究了干扰素诱导蛋白 35 (IFI35) 对 SHVV 复制的影响。IFI35 的过表达或抑制显著促进或降低了 SSN-1 细胞中 SHVV 的复制水平，这表明 IFI35 可能是 SSN-1 细胞中 SHVV 复制的一个正因子。我们的研究结果提供了一些有价值的信息，这将有助于未来对 SHVV-宿主相互作用的研究。
