Liu Xiaodan, Wen Yi, Hu Xianqin, Wang Wenwen, Liang Xufang, Li Jun, Vakharia Vikram, Lin Li
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
J Gen Virol. 2015 Apr;96(Pt 4):775-781. doi: 10.1099/vir.0.000037. Epub 2014 Dec 23.
Members of the genus Vesiculovirus, which belongs to the family Rhabdoviridae, can cause great economic loss in fish culture. In the present report, a vesiculovirus [named snakehead fish vesiculovirus (SHVV)] was isolated from diseased hybrid snakehead fish. SHVV shared 94 % nucleotide sequence identity at the genomic level with Siniperca chuatsi rhabdovirus (SCRV), which infects mandarin fish (S. chuatsi). We showed that SHVV was able to replicate and proliferate well in SSN-1 cells, which originate from striped snakehead fish (Channa striatus). Furthermore, mandarin fish was susceptible to SHVV by bath exposure, as well as by intraperitoneal injection. The infected fish showed typical clinical signs of rhabdovirus infection, including haemorrhage and oedema. Histopathological analysis revealed that extensive inflammation and necrosis were observed in the spleen, kidney, liver, heart and brain of the moribund mandarin fish. These results will shed new light on the epidemic of vesiculovirus infections among fish.
水泡病毒属属于弹状病毒科，其成员可在鱼类养殖中造成巨大经济损失。在本报告中，从患病的杂交乌鳢中分离出一种水泡病毒[命名为乌鳢水泡病毒（SHVV）]。SHVV在基因组水平上与感染鳜鱼（S. chuatsi）的鳜鱼弹状病毒（SCRV）具有94%的核苷酸序列同一性。我们发现SHVV能够在源自条纹乌鳢（Channa striatus）的SSN-1细胞中良好地复制和增殖。此外，鳜鱼通过浸浴暴露以及腹腔注射对SHVV易感。受感染的鱼表现出弹状病毒感染的典型临床症状，包括出血和水肿。组织病理学分析显示，濒死鳜鱼的脾脏、肾脏、肝脏、心脏和大脑中观察到广泛的炎症和坏死。这些结果将为鱼类中水泡病毒感染的流行提供新的线索。
