Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, 315800, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, 315800, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, 315800, China.
Fish Shellfish Immunol. 2019 Mar;86:70-81. doi: 10.1016/j.fsi.2018.11.030. Epub 2018 Nov 14.
Upon recognition of pathogen-associated molecular patterns by pattern-recognition receptors, immune cells are recruited, and multiple antibacterial/viral signaling pathways are activated, leading to the production of immune-related cytokines, chemokines, and interferons along with further activation of the adaptive immune response. MicroRNAs (miRs) play essential roles in regulating such immune signaling pathways, as well as the biological activities of immune cells; however, knowledge regarding the roles of miRs in the immune-related function of monocytes/macrophages (MO/MΦ) remains limited in teleosts. In the present study, we addressed the effects of miR-155 on Vibrio anguillarum-infected MO/MΦ. Our results showed that miR-155 augmented MO/MΦ expression of proinflammatory cytokines and attenuated the expression of anti-inflammatory cytokines. Additionally, the phagocytosis and bacteria-killing abilities of these cells were boosted by miR-155 administration, which also promoted M1-type polarization but inhibited M2-type polarization. Furthermore, the V. anguillarum-infection-induced apoptosis was also enhanced by miR-155 mimic transfection, which might have been due to excessive inflammation or the accumulation of reactive oxygen species. These results represent the first report providing a detailed account of the regulatory roles of miR-155 on MO/MΦ functions in teleosts and offer insight into the evolutionary history of miR-155-mediated regulation of host immune responses.
当模式识别受体识别病原体相关分子模式时，免疫细胞被募集，并且多种抗细菌/抗病毒信号通路被激活，导致免疫相关细胞因子、趋化因子和干扰素的产生，以及适应性免疫反应的进一步激活。microRNAs（miRs）在调节这些免疫信号通路以及免疫细胞的生物学活性方面发挥着重要作用；然而，miRs 在鱼类单核细胞/巨噬细胞（MO/MΦ）的免疫相关功能中的作用的知识仍然有限。在本研究中，我们研究了 miR-155 对鳗弧菌感染的 MO/MΦ 的影响。我们的结果表明，miR-155 增强了 MO/MΦ 促炎细胞因子的表达，并减弱了抗炎细胞因子的表达。此外，miR-155 的给药增强了这些细胞的吞噬和杀菌能力，促进了 M1 型极化，但抑制了 M2 型极化。此外，miR-155 模拟物转染还增强了鳗弧菌感染诱导的细胞凋亡，这可能是由于过度炎症或活性氧的积累。这些结果代表了第一个详细描述 miR-155 对鱼类 MO/MΦ 功能调节作用的报告，并深入了解了 miR-155 介导的宿主免疫反应调节的进化历史。
