Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
School of Medicine, Yangzhou Polytechnic College, Yangzhou 225009, China.
Bioresour Technol. 2019 Feb;273:196-202. doi: 10.1016/j.biortech.2018.10.085. Epub 2018 Oct 31.
Lysobacter enzymogenes OH11 is currently considered to be a novel biocontrol agent for various plant fungi diseases. At present, only heat-stable antifungal factor (HSAF) has been isolated and identified in culture, although other active compounds also showed antifungal activity. In the present study, a novel active compound, alteramide B (ATB), which exhibits broad-spectrum antagonistic activity against phytopathogenic fungi and oomycetes, was isolated. The genes responsible for ATB biosynthesis were also determined. In addition, a strain producing ATB with minimal HSAF production was successfully generated by redirecting metabolic flux, namely L. enzymogenes OH57. Furthermore, ATB production increased to 893.32 ± 15.57 mg/L through medium optimization and precursor supply strategy, which was 24.36-fold higher than that of 10% tryptic soy broth (36.67 ± 1.63 mg/L). Taken together, this study indicates ATB has great development value as a biopesticide because of its bioactivity and high production.
解淀粉芽胞杆菌 OH11 目前被认为是一种新型的植物真菌病害生物防治剂。目前，尽管其他活性化合物也显示出抗真菌活性，但仅在培养物中分离和鉴定了热稳定的抗真菌因子（HSAF）。在本研究中，分离到一种新型活性化合物，变曲菌素 B（ATB），它对植物病原真菌和卵菌具有广谱拮抗活性。还确定了负责 ATB 生物合成的基因。此外，通过重新定向代谢通量成功生成了产生 ATB 且 HSAF 产量最小的菌株，即解淀粉芽胞杆菌 OH57。此外，通过培养基优化和前体供应策略，ATB 的产量增加到 893.32 ± 15.57 mg/L，比 10%胰蛋白胨大豆肉汤（36.67 ± 1.63 mg/L）高 24.36 倍。综上所述，由于其生物活性和高产量，ATB 作为生物农药具有巨大的开发价值。
