疟原虫流行种群中 K13-螺旋桨基因多态性：负担和相关因素的系统评价方案。

K13-propeller gene polymorphisms in Plasmodium falciparum parasite population: a systematic review protocol of burden and associated factors.

机构信息

Department of Pharmacology & Therapeutics, Makerere University, P.O. Box 7072, Kampala, Uganda.

Department of Psychiatry, Makerere University, P.O. Box 7072, Kampala, Uganda.

出版信息

Syst Rev. 2018 Nov 17;7(1):199. doi: 10.1186/s13643-018-0866-7.

DOI:10.1186/s13643-018-0866-7

PMID:30447701

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6240191/

Abstract

BACKGROUND

Malaria control and prevention efforts continue to rely heavily on the use of medicines especially artemisinin agents. However, currently, the emergence of artemisinin resistance threatens this effort globally. The K13-gene polymorphisms associated with artemisinin resistance have been detected in Southeast Asia. In countries outside Southeast Asia, artemisinin resistance has not yet been confirmed.

METHODS/DESIGN: The articles will be obtained from the search of MEDLINE via PubMed, Scopus, EMBASE, and LILACS/VHL databases. Mesh terms will be used in the article search. Boolean operators ("AND", "OR") will be used in the article search. Article search will be done independently by two librarians (RS and AK). The articles will be screened for inclusion using set criteria and following the PRISMA guidelines. Data extraction will be done by two independent reviewers (NL and BB), Kappa statistic will be calculated, and any discrepancies resolved by discussion. Heterogeneity in the articles will be established using I statistic.

DISCUSSION

This review will focus on establishing the K13-gene polymorphisms among Plasmodium falciparum parasites reported from previous studies in malaria-affected countries. Artemisinin resistance has not been widely reported among parasites in Africa and other malaria-endemic countries outside Southeast Asia. However, several studies on artemisinin resistance have reported different K13-gene polymorphisms from the validated mutations found in Southeast Asia. This study will collate evidence from previous studies on the commonly reported K13 -gene polymorphisms among P. falciparum parasites in malaria-affected countries.

SYSTEMATIC REVIEW REGISTRATION

PROSPERO CRD 42018084624.

摘要

背景

疟疾防控工作仍然严重依赖药物，尤其是青蒿素类药物。然而，目前青蒿素耐药性的出现对全球防控工作构成了威胁。与青蒿素耐药性相关的 K13 基因突变已在东南亚国家检测到。在东南亚以外的国家，尚未确认存在青蒿素耐药性。

方法/设计：将通过 MEDLINE 搜索 PubMed、Scopus、EMBASE 和 LILACS/VHL 数据库获取相关文献。将使用 MeSH 术语进行文章检索。将使用布尔运算符（“AND”、“OR”）进行文章检索。两位图书管理员（RS 和 AK）将独立进行文章检索。将根据既定标准和 PRISMA 指南筛选文章是否纳入。将由两位独立评审员（NL 和 BB）进行数据提取，计算 Kappa 统计量，并通过讨论解决任何分歧。将使用 I 统计量评估文章的异质性。

讨论

本综述将重点关注先前在疟疾流行国家的研究中报道的恶性疟原虫寄生虫中的 K13 基因突变。在非洲和东南亚以外的其他疟疾流行国家，寄生虫中的青蒿素耐药性尚未广泛报道。然而，一些关于青蒿素耐药性的研究报告了与在东南亚发现的已验证突变不同的 K13 基因突变。本研究将整理先前研究中关于疟疾流行国家恶性疟原虫寄生虫中常见 K13 基因突变的证据。