IVF and Infertility Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 4941492, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
IVF and Infertility Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 4941492, Israel; Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Reprod Biomed Online. 2019 Jan;38(1):7-11. doi: 10.1016/j.rbmo.2018.10.002. Epub 2018 Nov 10.
Does delayed maturation of aspirated metaphase I (MI) oocytes, completed in vitro, adversely affect early embryo development?
Time-lapse microscopy was used to compare morphokinetic variables between embryos derived from oocytes with delayed maturation after ovarian stimulation and from in-vivo-matured metaphase II (MII) sibling oocytes from the same IVF and intracytoplasmic sperm injection cycle.
A total of 1545 injected oocytes in 169 cycles from 149 patients were included. The in-vitro-matured oocytes had lower normal fertilization rates than the MII aspirated oocytes (50.2% versus 68.0%; P < 0.001). Early key developmental events were significantly delayed in the normally fertilized in-vitro-matured compared with in-vivo-matured oocytes (polar body extrusion: 5.4 ± 3 versus 3.9 ± 1.8 h; P < 0.001; pronuclear fading: 27.2 ± 4.7 versus 25.1 ± 4.2 h; P < 0.001, respectively) and synchrony of the second cell cycle was adversely affected. The proportions of embryos with optimal second cell cycle length and second cell cycle were similar but with fewer top-quality embryos, based on an algorithm, for the delayed in-vitro-matured oocytes compared with their in-vivo-matured sibling oocytes (14% versus 29.1%; P < 0.001).
Embryos derived from oocytes that failed to mature in-vivo in standard treatment after ovarian stimulation may show a different morphokinetic profile from their sibling oocytes aspirated at the MII stage after completing maturation in-vivo.
体外培养的成熟延迟的抽吸中期 I（MI）卵母细胞是否会对早期胚胎发育产生不利影响？
使用时差显微镜比较来自卵巢刺激后成熟延迟的卵母细胞和来自同一体外受精和胞质内精子注射周期中体内成熟的中期 II（MII）姐妹卵母细胞的胚胎的形态动力学变量。
共纳入 149 名患者的 169 个周期中的 1545 个注射卵母细胞。体外成熟的卵母细胞的正常受精率低于 MII 抽吸卵母细胞（50.2%对 68.0%；P<0.001）。与体内成熟的卵母细胞相比，正常受精的体外成熟卵母细胞的早期关键发育事件明显延迟（极体排出：5.4±3 对 3.9±1.8 小时；P<0.001；原核消失：27.2±4.7 对 25.1±4.2 小时；P<0.001），并且第二细胞周期的同步性受到不利影响。与体内成熟的姐妹卵母细胞相比，延迟体外成熟的卵母细胞的胚胎具有最佳第二细胞周期长度和第二细胞周期的比例相似，但根据算法，优质胚胎较少（14%对 29.1%；P<0.001）。
在卵巢刺激后的标准治疗中未能体内成熟的卵母细胞产生的胚胎可能与体内完成成熟后抽吸的 MII 期姐妹卵母细胞具有不同的形态动力学特征。
