Department of Orthopedics, West China Hospital, Sichuan University, Guoxuexiang, No.37, Chengdu 610041, Sichuan Province, People's Republic of China; Department of Emergency, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, People's Republic of China.
Department of Orthopedics, West China Hospital, Sichuan University, Guoxuexiang, No.37, Chengdu 610041, Sichuan Province, People's Republic of China.
Int J Biol Macromol. 2019 Mar 1;124:360-367. doi: 10.1016/j.ijbiomac.2018.11.132. Epub 2018 Nov 15.
In the present study, the pharmacological effects of oligosaccharides from Cistanche deserticola extract on inflammation, oxidative stress, and apoptosis in male albino rats with spinal cord injury were investigated. Lipid peroxidation, GSH, catalase, superoxide dismutase, acetylcholine esterase, GPx, ROS, and nitric acid were significantly altered in the rats with spinal cord injury. The mRNA expression levels of IL-6, TNF-α, cyclooxygenase-2, iNOS, p53, caspase-3, bax, and pro-NGF were reduced by >20% following extract supplementation. Protein expression levels of caspase-3 and pro-NGF were also reduced by >20%. The number of p53 positive cells was 1, 79, 54, 33, and 19 in groups GI-GV, respectively, and the corresponding numbers of caspase-3 positive cells were 2, 87, 51, 23, and 14. Based on the present results, the use of oligosaccharides from Cistanche deserticola extract was effective against inflammation, oxidative stress, and apoptosis in spinal cord injury male albino rats.
在本研究中，研究了肉苁蓉提取物寡糖对脊髓损伤雄性白化大鼠炎症、氧化应激和细胞凋亡的药理作用。脊髓损伤大鼠的脂质过氧化、GSH、过氧化氢酶、超氧化物歧化酶、乙酰胆碱酯酶、GPx、ROS 和硝酸水平发生了显著变化。提取物补充后，IL-6、TNF-α、环氧化酶-2、iNOS、p53、caspase-3、Bax 和 pro-NGF 的 mRNA 表达水平降低了>20%。caspase-3 和 pro-NGF 的蛋白表达水平也降低了>20%。各组 p53 阳性细胞数分别为 GI-GV 组的 1、79、54、33 和 19，相应的 caspase-3 阳性细胞数分别为 2、87、51、23 和 14。基于目前的结果，肉苁蓉提取物寡糖可有效对抗雄性白化大鼠脊髓损伤引起的炎症、氧化应激和细胞凋亡。
