State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering & Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, China.
Colloids Surf B Biointerfaces. 2019 Feb 1;174:161-167. doi: 10.1016/j.colsurfb.2018.11.019. Epub 2018 Nov 10.
Phenolic compounds can act as protective agents against free radical oxidation. It is desirable to develop simple and efficient analytical techniques to measure the phenolic content and their antioxidant capacity. Using Enteromorpha prolifera (EP) as the carbon source, novel fluorescent carbon dots (EP-CDs) were synthesized through a facile microwave-assisted hydrothermal method. EP-CDs exhibit high mono-dispersity in aqueous solution, low cytotoxicity, and dual fluorescence emission in the visible and near-infrared range. The fluorescence of EP-CDs is inert to phenolic compounds and hydroxyl free radicals, but could be rapidly and efficiently quenched by the quinoid oxidation products. By monitoring the fluorescence quenching of EP-CDs, it is demonstrated that the radical scavenging processes of different natural phenolic compounds could be sensitively detected. By employing this eco-friendly and economic analytical method, phenols with different structure or concentration could be rapidly detected and determined. EP-CDs also have potential for additional applications, such as hydrogen peroxide and iron analysis.
酚类化合物可以作为自由基氧化的保护剂。因此，开发简单高效的分析技术来测量酚类物质的含量及其抗氧化能力是非常有必要的。本文以浒苔（Enteromorpha prolifera，EP）为碳源，通过简便的微波辅助水热法合成了新型荧光碳点（EP-CDs）。EP-CDs 在水溶液中具有高单分散性、低细胞毒性和可见及近红外区的双荧光发射。EP-CDs 的荧光对酚类化合物和羟基自由基不敏感，但可以被醌型氧化产物快速有效地猝灭。通过监测 EP-CDs 的荧光猝灭，可以证明可以灵敏地检测不同天然酚类化合物的自由基清除过程。通过采用这种环保且经济的分析方法，可以快速检测和定量不同结构或浓度的酚类物质。EP-CDs 还具有用于分析过氧化氢和铁等物质的潜力。
