College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China.
Chemosphere. 2019 Feb;217:646-658. doi: 10.1016/j.chemosphere.2018.11.070. Epub 2018 Nov 12.
Microplastics are major pollutants in marine environment and may have health effects on aquatic organisms. In this study, we used two sizes (5 and 50 μm diameter) of fluorescent and virgin polystyrene microplastics to analyze the adverse effects on larval zebrafish. In our study, we evaluated the effects on larval zebrafish after exposure to 100 and 1000 μg/L of two sizes of polystyrene microplastics for 7 days. Our results show that polystyrene microplastics could cause alterations in the microbiome at the phylum and genus levels in larval zebrafish, including changes in abundance and diversity of the microbiome. In addition, metabolomic analysis suggested that exposure to polystyrene microplastics induced alterations of metabolic profiles in larval zebrafish, and differential metabolites were involved in energy metabolism, glycolipid metabolism, inflammatory response, neurotoxic response, nucleic acid metabolism, oxidative stress. Polystyrene microplastics also significantly decreased the activities of catalase and the content of glutathione. In addition, the results of gene transcription analysis showed that exposure to polystyrene microplastics induced changes in glycolysis-related genes and lipid metabolism-related genes, confirming that polystyrene microplastics disturbed glycolipid and energy metabolism. Taken together, the results obtained in the present study indicated that the potential effects of environmental microplastics on aquatic organisms should not be ignored.
微塑料是海洋环境中的主要污染物，可能对水生生物产生健康影响。在本研究中，我们使用两种尺寸（直径 5 和 50 μm）的荧光和原始聚苯乙烯微塑料来分析其对幼斑马鱼的不良影响。在我们的研究中，我们评估了暴露于 100 和 1000μg/L 两种尺寸的聚苯乙烯微塑料 7 天后对幼斑马鱼的影响。我们的结果表明，聚苯乙烯微塑料可能会导致幼斑马鱼的微生物组在门和属水平上发生改变，包括微生物组的丰度和多样性的变化。此外，代谢组学分析表明，暴露于聚苯乙烯微塑料会诱导幼斑马鱼代谢谱的改变，差异代谢物涉及能量代谢、糖脂代谢、炎症反应、神经毒性反应、核酸代谢、氧化应激。聚苯乙烯微塑料还显著降低了过氧化氢酶的活性和谷胱甘肽的含量。此外，基因转录分析的结果表明，暴露于聚苯乙烯微塑料会诱导与糖酵解相关的基因和与脂质代谢相关的基因发生变化，证实了聚苯乙烯微塑料干扰了糖脂和能量代谢。综上所述，本研究的结果表明，环境微塑料对水生生物的潜在影响不容忽视。
