State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension, Department of Hypertension, Ruijin Hospital and Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Key Laboratory of Stem Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Cell Mol Life Sci. 2019 Feb;76(4):777-789. doi: 10.1007/s00018-018-2970-1. Epub 2018 Nov 17.
Thoracic aorta perivascular adipose tissue (T-PVAT) has critical roles in regulating vascular homeostasis. However, the developmental characteristics and cellular lineage of adipocyte in the T-PVAT remain unclear. We show that T-PVAT contains three long strip-shaped fat depots, anterior T-PVAT (A-T-PVAT), left lateral T-PVAT (LL-T-PVAT), and right lateral T-PVAT (RL-T-PVAT). A-T-PVAT displays a distinct transcriptional profile and developmental origin compared to the two lateral T-PVATs (L-T-PVAT). Lineage tracing studies indicate that A-T-PVAT adipocytes are primarily derived from SM22α progenitors, whereas L-T-PVAT contains both SM22α and Myf5 cells. We also show that L-T-PVAT contains more UCP1 brown adipocytes than A-T-PVAT, and L-T-PVAT exerts a greater relaxing effect on aorta than A-T-PVAT. Angiotensin II-infused hypertensive mice display greater macrophage infiltration into A-T-PVAT than L-T-PVAT. These combined results indicate that L-T-PVAT has a distinct development from A-T-PVAT with different cellular lineage, and suggest that L-T-PVAT and A-T-PVAT have different physiological and pathological functions.
胸主动脉周围脂肪组织（T-PVAT）在调节血管稳态方面具有重要作用。然而，T-PVAT 中脂肪细胞的发育特征和细胞谱系仍不清楚。我们发现 T-PVAT 包含三个长条状的脂肪垫，即前 T-PVAT（A-T-PVAT）、左外侧 T-PVAT（LL-T-PVAT）和右外侧 T-PVAT（RL-T-PVAT）。A-T-PVAT 与两个外侧 T-PVAT（L-T-PVAT）相比，具有明显不同的转录谱和发育起源。谱系追踪研究表明，A-T-PVAT 脂肪细胞主要来源于 SM22α 前体细胞，而 L-T-PVAT 则包含 SM22α 和 Myf5 细胞。我们还发现，L-T-PVAT 比 A-T-PVAT 含有更多的 UCP1 棕色脂肪细胞，并且 L-T-PVAT 对主动脉的舒张作用强于 A-T-PVAT。血管紧张素 II 输注的高血压小鼠显示 A-T-PVAT 中有更多的巨噬细胞浸润。这些综合结果表明，L-T-PVAT 与 A-T-PVAT 具有不同的发育和细胞谱系，提示 L-T-PVAT 和 A-T-PVAT 具有不同的生理和病理功能。
