Xing Xiaojuan, Jiang Jiafu, Huang Yaoyao, Zhang Zixin, Song Aiping, Ding Lian, Wang Haibing, Yao Jianjun, Chen Sumei, Chen Fadi, Fang Weimin
Key Laboratory of Landscape Agriculture, College of Horticulture, Ministry of Agriculture, Nanjing Agricultural University, Weigang No. 1, Nanjing, 210095, Jiangsu, Republic of China.
Shanghai Honghua Horticulture Co. Ltd., Shanghai, 200070, China.
Mol Biotechnol. 2019 Jan;61(1):20-31. doi: 10.1007/s12033-018-0134-z.
AP2/ERF transcription factors (TFs) represent valuable targets for the genetic manipulation of crop plants, as they participate in the control of metabolism, growth and development, as well as in the plants' response to environmental stimuli. Here, an ERF TF encoded by the chrysanthemum (Chrysanthemum morifolium) genome, designated CmERF110, was cloned and functionally characterized. The predicted CmERF110 polypeptide included a conserved DNA-binding AP2/ERF domain. A transient expression experiment revealed that the protein was deposited in the nucleus, and a transactivation experiment in yeast suggested that it had no transcriptional activity. The gene was transcribed in the chrysanthemum root, stem and leaf, with its transcript level following a circadian rhythm under both long and short days. The effect of constitutively expressing the gene in Arabidopsis thaliana was to accelerate flowering. Transcriptional profiling implied that its effect on floral initiation operated through the photoperiod pathway.
AP2/ERF转录因子（TFs）是作物遗传操作的重要靶点，因为它们参与调控新陈代谢、生长发育，以及植物对环境刺激的响应。本文克隆并鉴定了菊花（Chrysanthemum morifolium）基因组编码的一个ERF转录因子，命名为CmERF110。预测的CmERF110多肽包含一个保守的DNA结合AP2/ERF结构域。瞬时表达实验表明该蛋白定位于细胞核，酵母中的反式激活实验表明它没有转录活性。该基因在菊花的根、茎和叶中均有转录，其转录水平在长日照和短日照条件下均呈现昼夜节律。在拟南芥中组成型表达该基因可加速开花。转录谱分析表明其对开花起始的影响是通过光周期途径实现的。
