Sun Jing, Wang Heng, Ren Liping, Chen Sumei, Chen Fadi, Jiang Jiafu
College of Horticulture, Nanjing Agricultural University , Nanjing 210095, China.
Hortic Res. 2017 Feb 15;4:17001. doi: 10.1038/hortres.2017.1. eCollection 2017.
The chrysanthemum genome harbors three genes: and are thought to act as regulators of floral induction under long-day (LD) and short-day (SD) conditions, respectively, whereas the function of is currently unclear. The objective of the present research was to explore the function of in the determination of flowering time of the photo-insensitive chrysanthemum cultivar 'Floral Yuuka', both in response to variation in the photoperiod and to the exogenous provision of sucrose. Spraying leaves of 'Floral Yuuka' plants with 50 mM sucrose accelerated flowering and increased the level of transcription in the leaf more strongly than either or under both long and SD conditions. Transcription profiling indicated that all three genes were upregulated during floral induction. The relationship of the sequence with that of other members of the family suggested that its product contributes to the florigen rather than to the anti-florigen complex. The heterologous expression of in the mutant rescued the mutant phenotype, showing that could compensate for the absence of . These results suggest that acts as a regulator of floral transition and responds to both the photoperiod and sucrose.
[基因名称1]和[基因名称2]被认为分别在长日照（LD）和短日照（SD）条件下作为成花诱导的调节因子，而[基因名称3]的功能目前尚不清楚。本研究的目的是探究[基因名称3]在光不敏感菊花品种‘Floral Yuuka’开花时间决定中的作用，包括对光周期变化和外源蔗糖供应的响应。在长日照和短日照条件下，用50 mM蔗糖喷洒‘Floral Yuuka’植株的叶片均加速了开花，且比[基因名称1]或[基因名称2]更强烈地增加了叶片中[基因名称3]的转录水平。转录谱分析表明，在成花诱导过程中所有三个[基因名称]基因均上调。[基因名称3]序列与[基因家族名称]其他成员序列的关系表明，其产物有助于成花素而非抗成花素复合体。[基因名称3]在[突变体名称]突变体中的异源表达挽救了突变体表型，表明[基因名称3]可以补偿[基因名称1]的缺失。这些结果表明，[基因名称3]作为成花转变的调节因子，对光周期和蔗糖均有响应。
