用于扩展景深显微镜的合成贝塞尔光针。
Synthetic Bessel light needle for extended depth-of-field microscopy.
作者信息
Yang Jiamiao, Gong Lei, Shen Yuecheng, Wang Lihong V
机构信息
Caltech Optical Imaging Laboratory, Andrew and Peggy Cherng Department of Medical Engineering, Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, Anhui 230026, China.
出版信息
Appl Phys Lett. 2018 Oct 29;113(18):181104. doi: 10.1063/1.5058163. Epub 2018 Nov 2.
An ultra-long light needle is highly desired in optical microscopy for its ability to improve the lateral resolution over a large depth of field (DOF). However, its use in image acquisition usually relies on mechanical raster scanning, which compromises between imaging speed and stability and thereby restricts imaging performance. Here, we propose a synthetic Bessel light needle (SBLN) that can be generated and scanned digitally by complex field modulation using a digital micromirror device. In particular, the SBLN achieves a 45-fold improvement in DOF over its counterpart Gaussian focus. Further, we apply the SBLN to perform motionless two-dimensional and three-dimensional microscopic imaging, achieving both improved resolution and extended DOF. Our work is expected to open up opportunities for potential biomedical applications.
在光学显微镜中，超长光针因其能够在大景深（DOF）范围内提高横向分辨率而备受青睐。然而，其在图像采集过程中的应用通常依赖于机械光栅扫描，这在成像速度和稳定性之间存在权衡，从而限制了成像性能。在此，我们提出一种合成贝塞尔光针（SBLN），它可以通过使用数字微镜器件进行复场调制以数字方式生成和扫描。特别是，SBLN在景深方面比其对应的高斯焦点提高了45倍。此外，我们应用SBLN进行静止的二维和三维显微成像，实现了分辨率的提高和景深的扩展。我们的工作有望为潜在的生物医学应用开辟机会。
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