Gong Sanqiang, Chai GuangJun, Xiao Yilin, Xu Lijia, Yu Kefu, Li Jinlong, Liu Fang, Cheng Hao, Zhang Fengli, Liao Baolin, Li Zhiyong
Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Coral Reef Research Center of China, Guangxi University, Nanning, China.
Front Microbiol. 2018 Nov 2;9:2485. doi: 10.3389/fmicb.2018.02485. eCollection 2018.
The coral symbiont plays important roles in the adaptation of coral to environmental changes. However, coral- symbiotic associations are not well-understood in the South China Sea (SCS) whilst considering environmental factors and host taxa. In this study, next-generation sequencing of the internal transcribed spacer region 2 (ITS2) marker gene was used to explore the symbiotic associations between and five typical coral species across tropical and subtropical reef regions of the SCS. The results showed that sp., , , and exhibited distinct compositions between tropical and subtropical reef regions, whereas had stable compositions. More heterogeneous compositions among different coral species were observed in the tropical region, but there were no statistically significant differences in compositions among different coral species in subtropical reef regions. There was a correlation between the compositions and environmental factors, except for the composition of . subclades D1, D2, C71, C71a, C21, C3b, and C161 were primarily explained by the seawater temperature, nitrate, ammonia, and phosphate. Several host-specific subclades (e.g., C15, C15.6, and C91) were observed in as well. The findings of this study demonstrate the relationship of diversity with coral hosts and the environment are helpful for elucidating the adaptation of corals to global climate change and anthropogenic disturbance.
珊瑚共生体在珊瑚适应环境变化中发挥着重要作用。然而，在考虑环境因素和宿主分类群的情况下，南海的珊瑚 - 共生体关系尚未得到充分了解。在本研究中，利用内部转录间隔区2（ITS2）标记基因的二代测序技术，探究了南海热带和亚热带珊瑚礁区域的[共生体名称]与五种典型珊瑚物种之间的共生关系。结果表明，[共生体名称1]、[共生体名称2]、[共生体名称3]、[共生体名称4]在热带和亚热带珊瑚礁区域呈现出不同的[共生体名称]组成，而[共生体名称5]具有稳定的[共生体名称]组成。在热带区域，不同珊瑚物种之间观察到更具异质性的[共生体名称]组成，但在亚热带珊瑚礁区域，不同珊瑚物种之间的[共生体名称]组成没有统计学上的显著差异。除了[共生体名称6]的组成外，[共生体名称]组成与环境因素之间存在相关性。[共生体名称]的D1、D2、C71、C71a、C21、C3b和C161亚分支主要受海水温度、硝酸盐、氨和磷酸盐的影响。在[珊瑚物种名称]中也观察到了几个宿主特异性的[共生体名称]亚分支（例如C15、C15.6和C91）。本研究结果表明[共生体名称]多样性与珊瑚宿主和环境之间的关系，有助于阐明珊瑚对全球气候变化和人为干扰的适应性。
