HipA介导的SeqA磷酸化不影响……中的复制起始。（原文句末不完整，翻译时保留了原文的省略状态）

HipA-Mediated Phosphorylation of SeqA Does not Affect Replication Initiation in .

作者信息

Riber Leise, Koch Birgit M, Kruse Line Riis, Germain Elsa, Løbner-Olesen Anders

机构信息

Section for Functional Genomics, Department of Biology, Center for Bacterial Stress Response and Persistence, University of Copenhagen, Copenhagen, Denmark.

Laboratoire de Chimie Bactérienne, Université Aix-Marseille, CNRS, Marseille, France.

出版信息

Front Microbiol. 2018 Nov 2;9:2637. doi: 10.3389/fmicb.2018.02637. eCollection 2018.

DOI:10.3389/fmicb.2018.02637

PMID:30450091

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6225831/

Abstract

The SeqA protein of is required to prevent immediate re-initiation of chromosome replication from . The SeqA protein is phosphorylated at the serine-36 (Ser36) residue by the HipA kinase. The role of phosphorylation was addressed by mutating the Ser36 residue to alanine, which cannot be phosphorylated and to aspartic acid, which mimics a phosphorylated serine residue. Both mutant strains were similar to wild-type with respect to origin concentration and initiation synchrony. The minimal time between successive initiations was also unchanged. We therefore suggest that SeqA phosphorylation at the Ser36 residue is silent, at least with respect to SeqA's role in replication initiation.

摘要

需SeqA蛋白来防止染色体复制从……处立即重新起始。SeqA蛋白在丝氨酸36（Ser36）残基处被HipA激酶磷酸化。通过将Ser36残基突变为无法磷酸化的丙氨酸以及模拟磷酸化丝氨酸残基的天冬氨酸来探讨磷酸化的作用。两种突变菌株在起始点浓度和起始同步性方面与野生型相似。连续起始之间的最短时间也未改变。因此，我们认为Ser36残基处的SeqA磷酸化是无活性的，至少就SeqA在复制起始中的作用而言。