Purification of Rts1 RepA protein and binding of the protein to mini-Rts1 DNA.
作者信息
Kamio Y, Itoh Y, Terawaki Y
机构信息
Department of Bacteriology, Shinshu University School of Medicine, Matsumoto, Japan.
出版信息
J Bacteriol. 1988 Sep;170(9):4411-4. doi: 10.1128/jb.170.9.4411-4414.1988.
Abstract
RepA protein, essential for the replication of plasmid Rts1, was purified, and its binding to mini-Rts1 subregions was examined by a DNase I protection assay. RepA protected the incI and incII iterons, a region immediately upstream of the repA promoter, and a 10-base-pair region located between the most external incII iteron and a GATC box. The protection was less efficient when preheated RepA was used.
摘要
Zotero 插件