a South Asian Clinical Toxicology Research Collaboration, Faculty of Medicine , University of Peradeniya , Peradeniya , Sri Lanka.
c Discipline of Pharmacology, School of Medical Sciences , The University of Sydney , Sydney , New South Wales, Australia.
Clin Toxicol (Phila). 2019 Jun;57(6):411-414. doi: 10.1080/15563650.2018.1530778. Epub 2018 Nov 17.
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are used in clinical management to confirm the diagnosis and indicate the severity of organophosphorus and carbamate poisoning. ChE check mobile is a new portable cholinesterase testing system developed in Germany. The study aims to evaluate the accuracy of ChE check mobile compared to the standard reference method and Test-mate ChE system.
Patients with organophosphorus and carbamate poisoning were recruited from two general hospitals in Sri Lanka between September 2013 and November 2014. The AChE was measured using the three methods.
Blood samples were collected from 185 self-poisoned patients (170 organophosphorus and 15 carbamate) and 20 normal individuals. ChE check mobile correlated well with spectrophotometer readings (Pearson's correlation coefficient 0.87) but gave higher values (Mean bias for AChE: +6.55 (95% CI: -11 to 24) U/g Hb). A similar positive bias from Test-mate results was also observed. Applying a correction factor derived from the volunteer samples (dividing by 1.353) greatly improved agreement in pesticide poisoned patients.
ChE check mobile system allowed for rapid determination of AChE activity but gave somewhat higher AChE compared to other methods. Applying a correction factor of 1.353 provide a good agreement to both reference and Test-mate ChE machine in this setting.
乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）用于临床管理，以确认诊断并指示有机磷和氨基甲酸酯中毒的严重程度。ChE check mobile 是德国开发的一种新型便携式胆碱酯酶检测系统。本研究旨在评估 ChE check mobile 与标准参考方法和 Test-mate ChE 系统相比的准确性。
2013 年 9 月至 2014 年 11 月，在斯里兰卡的两家综合医院招募了有机磷和氨基甲酸酯中毒的患者。使用三种方法测量 AChE。
采集了 185 名自服中毒患者（170 名有机磷中毒和 15 名氨基甲酸酯中毒）和 20 名正常个体的血样。ChE check mobile 与分光光度计读数相关性良好（Pearson 相关系数 0.87），但给出的数值较高（AChE 的平均偏差：+6.55（95%CI：-11 至 24）U/gHb）。Test-mate 结果也观察到类似的正偏差。应用志愿者样本得出的校正因子（除以 1.353）可显著提高在农药中毒患者中的一致性。
ChE check mobile 系统允许快速测定 AChE 活性，但与其他方法相比，AChE 略高。在这种情况下，应用 1.353 的校正因子可以与参考和 Test-mate ChE 机器达成良好的一致性。
