1Departamento de Fisiología,Universidad de la República,Lasplaces 1620,Montevideo 11600,Uruguay.
2Departamento de Farmacología,Universidad de la República,Lasplaces 1620,Montevideo 11600,Uruguay.
Animal. 2018 Dec;12(12):2598-2608. doi: 10.1017/S1751731118000320.
Electroejaculation (EE) is stressful and probably painful; thus the administration of anaesthesia is recommended to decrease those negative effects. However, anaesthesia has a direct risk of provoking death, but sedation is less risky than anaesthesia. At the same time, α2-adrenergic agonists may improve semen quality. Therefore, the aim of the present study was to compare the physiological and behavioural responses indicative of stress and possibly pain, and the semen quality in electroejaculated untreated, anaesthetised or sedated goat bucks. Semen was collected from eight bucks using three different procedures in all them (EE in untreated bucks, EE under sedation or EE under general anaesthesia). The number of vocalizations during EE and the behavioural pattern before and after procedures were recorded. Pain visual analogue scale (VAS) score was also determined during EE. Rectal temperature, heart rate, serum cortisol concentration, biochemical and haematological parameters were measured before and after each procedure, and sperm characteristics were determined. Bucks vocalised more often when untreated than sedated or anaesthetised (P<0.02). The pain VAS score was greater when bucks were untreated than sedated or anaesthetised (P<0.002). The rectal temperature, heart rate, total protein, albumin and haemoglobin concentrations were greater when bucks were untreated than anaesthetised or sedated (P<0.02). Serum cortisol increased after EE (P=0.0006), without differences between procedures. The frequency and duration of lying down after EE were greater when bucks were anaesthetised than sedated or untreated (P<0.05), and were also greater when bucks were sedated than untreated (P<0.05). The number of times that the animal tried to stand up after EE was greater when bucks were anaesthetised than sedated or untreated (P<0.02). The sperm mass motility was greater when bucks were anaesthetised or sedated than when they were untreated (P=0.048). When animals were sedated, the ejaculate contained more spermatozoa with functional plasma membrane (P=0.03) and morphologically normal (P=0.05) than when they were untreated. In conclusion, general anaesthesia and sedation decreased the stress and probably the pain response provoked by EE and especially sedation improved the quality of the semen collected.
电刺激射精（EE）会带来压力，而且可能很痛苦；因此，建议使用麻醉来减轻这些负面影响。然而，麻醉会直接带来死亡风险，而镇静的风险则小于麻醉。同时，α2 肾上腺素能激动剂可能会改善精液质量。因此，本研究的目的是比较未经处理、麻醉或镇静的山羊公鹿在电刺激射精时表明压力和可能疼痛的生理和行为反应，以及精液质量。使用三种不同的程序从八只公鹿中收集精液，所有这些程序都是在未经处理的公鹿中进行电刺激射精、在镇静下进行电刺激射精或在全身麻醉下进行电刺激射精。在电刺激射精期间记录了发声次数，以及在程序前后的行为模式。还在电刺激射精期间确定了疼痛视觉模拟评分（VAS）。在每次程序前后测量了直肠温度、心率、血清皮质醇浓度、生化和血液学参数，并确定了精子特征。与镇静或麻醉相比，未经处理的公鹿发声次数更多（P<0.02）。与镇静或麻醉相比，未经处理的公鹿的疼痛 VAS 评分更高（P<0.002）。与麻醉或镇静相比，未经处理的公鹿的直肠温度、心率、总蛋白、白蛋白和血红蛋白浓度更高（P<0.02）。电刺激射精后血清皮质醇增加（P=0.0006），但程序之间没有差异。麻醉后躺下的频率和持续时间大于镇静或未经处理（P<0.05），镇静后也大于未经处理（P<0.05）。电刺激射精后试图站起来的次数，麻醉后的公鹿多于镇静或未经处理的公鹿（P<0.02）。麻醉或镇静后的精子质量活力大于未经处理的公鹿（P=0.048）。当动物镇静时，与未经处理相比，精液中含有更多具有功能细胞膜的精子（P=0.03）和形态正常的精子（P=0.05）。总之，全身麻醉和镇静减轻了 EE 引起的应激反应，可能还减轻了疼痛反应，特别是镇静提高了采集的精液质量。
