Departamento de Biociencias Veterinarias, Facultad de Veterinaria Universidad de la República, Montevideo, Uruguay.
Departamento de Biociencias Veterinarias, Facultad de Veterinaria Universidad de la República, Montevideo, Uruguay.
Theriogenology. 2021 Sep 15;172:142-149. doi: 10.1016/j.theriogenology.2021.06.016. Epub 2021 Jun 20.
The cryoresistance of bucks' semen collected by transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) and electroejaculation (EE) was compared during the breeding season (Study 1) or the late non-breeding season (Study 2). Semen was collected from 10 Gabon bucks with both methods in each season and then frozen according to a standardized protocol. The time required for ejaculation, the number of electrical pulses applied, the sum of the electrical pulses applied∗voltage and the total number of vocalizations emitted during the collection by each animal were recorded. In Study 1, TUMASG required a longer time (P = 0.0006) but fewer electrical pulses and a lower sum of pulses∗voltage than EE (P < 0.0001 for both comparisons). Bucks vocalized fewer times during TUMASG than during EE (P < 0001). Semen collected with TUMASG had greater sperm concentration, sperm mass motility, total number of motile sperm and of sperm with progressive motility, and tended to have greater total number of sperm with functional membrane than semen collected with EE (P = 0.02; P = 0.003; P = 0.02; P = 0.02; P = 0.06, respectively). After the freezing-thawing process, sperm collected with TUMASG had a greater quality of the motility, a total number of motile sperm and sperm with progressive motility, a greater percentage of motile sperm and sperm with functional membrane, and tended to have a greater total number of sperm with normal morphology than semen collected with EE (P = 0.04; P = 0.04; P = 0.03; P = 0.02; P = 0.04; P = 0.06, respectively). In Study 1 sperm collected with TUMASG had greater cryoresistance for almost all the variables considered than when it was collected with EE. In Study 2, the number of electrical pulses and the sum of pulses∗voltage were greater with EE than with TUMASG (P < 0001; P = 0.0002, respectively). There were no differences in any sperm variable in fresh or thawed samples collected with TUMASG or EE. However, the other seminal characteristics evaluated did not differ according to the method, and there were no differences in the cryoresistance of any variable. Overall, TUMASG affected animal welfare less than EE and was suitable for collecting semen of good quality with high cryoresistance during the breeding season in buck.
比较了通过经直肠超声引导的副性腺按摩（TUMASG）和电刺激射精（EE）采集的雄鹿精液在繁殖季节（研究 1）或繁殖后期（研究 2）的抗冻能力。在每个季节，使用这两种方法从 10 只加蓬雄鹿中采集精液，然后按照标准化方案进行冷冻。记录了每种动物采集过程中射精所需的时间、应用的电脉冲数量、应用的电脉冲总和∗电压以及发出的总发声次数。在研究 1 中，TUMASG 需要的时间更长（P = 0.0006），但电脉冲数量更少，脉冲总和∗电压也低于 EE（两种比较均 P < 0.0001）。TUMASG 采集时，雄鹿发声次数少于 EE 采集时（P < 0.0001）。TUMASG 采集的精液精子浓度、精子质量活力、总活力精子数和具有前向运动的精子数较高，且具有功能膜的精子总数有较高的趋势，高于 EE 采集的精液（P = 0.02；P = 0.003；P = 0.02；P = 0.02；P = 0.06）。在冷冻-解冻过程后，TUMASG 采集的精子具有更好的运动质量、总活力精子数和具有前向运动的精子数、更高比例的活力精子和具有功能膜的精子，且具有正常形态的精子总数有较高的趋势，高于 EE 采集的精液（P = 0.04；P = 0.04；P = 0.03；P = 0.02；P = 0.04；P = 0.06）。在研究 1 中，与 EE 相比，TUMASG 采集的精子在几乎所有考虑的变量方面都具有更高的抗冻能力。在研究 2 中，EE 采集的电脉冲数量和脉冲总和∗电压均大于 TUMASG（P < 0001；P = 0.0002）。TUMASG 和 EE 采集的新鲜或解冻样本中，没有一个精子变量存在差异。然而，评估的其他精液特征不受方法影响，并且没有一个变量的抗冻能力存在差异。总体而言，TUMASG 对动物福利的影响小于 EE，并且适合在繁殖季节采集具有高抗冻能力的优质精液。
