College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
Chem Commun (Camb). 2018 Dec 4;54(97):13678-13681. doi: 10.1039/c8cc08553f.
We develop a new DNAzyme biosensor for Argonaute 2 (Ago2) assay based on target-initiated rolling circle amplification in combination with an 8-17 DNAzyme-mediated dual signal amplification strategy. This biosensor can achieve ultrahigh sensitivity with a detection limit of 0.35 pM, and it can be further used for the screening of Ago2 inhibitors and the accurate measurement of endogenous Ago2 from HeLa cell extracts.
我们开发了一种基于目标引发滚环扩增的新型 Argonaute 2 (Ago2) 分析物的 DNAzyme 生物传感器，结合 8-17 DNAzyme 介导的双重信号放大策略。该生物传感器具有超高的灵敏度，检测限低至 0.35 pM，可进一步用于 Ago2 抑制剂的筛选和 HeLa 细胞提取物中内源性 Ago2 的准确测量。
